Key features and details
- Mouse monoclonal [mAbcam77169] to TICAM2
- Suitable for: WB, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-TICAM2 antibody [mAbcam77169]
See all TICAM2 primary antibodies
DescriptionMouse monoclonal [mAbcam77169] to TICAM2
Tested applicationsSuitable for: WB, Flow Cytmore details
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human TICAM2 aa 200 to the C-terminus conjugated to keyhole limpet haemocyanin.
(Peptide available as
- Recombinant Human TICAM2 protein (ab123160) can be used as a positive control in WB. This antibody gave a positive signal in Human Kidney and Human Spleen Tissue Lyates
This antibody clone is manufactured by Abcam.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Concentration information loading...
Our Abpromise guarantee covers the use of ab77169 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 10 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 27 kDa).|
|Flow Cyt||Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
FunctionFunctions in LPS-TLR4 signaling to regulate the MYD88-independent pathway during the innate immune response to LPS. Also involved in IL1-triggered NF-kappa-B activation, functioning upstream of IRAK1, IRAK2, TRAF6, and IKBKB. Physically bridges TLR4 and TICAM1 and functionally transmits LPS-TRL4 signal to TICAM1.
Tissue specificityExpressed in spleen, prostate, testis, uterus, small intestine, colon, peripheral blood leukocytes, heart, placenta, lung, liver, skeletal muscle, and pancreas.
Sequence similaritiesContains 1 TIR domain.
DomainThe TIR domain mediates the interaction with TRAF6.
modificationsPhosphorylated by PKCE in response to LPS. Phosphorylation is essential for its function. It is depleted from the membrane upon phosphorylation.
Myristoylated. Required for membrane association which is critical for its ability to initiate efficient signaling.
Cellular localizationCytoplasm. Golgi apparatus. Cell membrane. Localized to the plasma membrane as a result of myristoylation. Phosphorylation on Ser-16 leads to its depletion from the membrane.
- Information by UniProt
- Putative NF-kappa-B-activating protein 502 antibody
- TCAM2_HUMAN antibody
- TICAM-2 antibody
All lanes : Anti-TICAM2 antibody [mAbcam77169] (ab77169) at 10 µg/ml
Lane 1 : Human kidney tissue lysate - total protein (ab30203)
Lane 2 : Human spleen tissue lysate - total protein (ab29699)
Lysates/proteins at 20 µg per lane.
All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 27 kDa
Observed band size: 32 kDa why is the actual band size different from the predicted?
Overlay histogram showing PC3 cells stained with ab77169 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab77169, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
ab77169 has not yet been referenced specifically in any publications.