Overview

  • Product name

    Anti-TICAM2 antibody [mAbcam77169]
    See all TICAM2 primary antibodies
  • Description

    Mouse monoclonal [mAbcam77169] to TICAM2
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human TICAM2 aa 200 to the C-terminus conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab57980)

  • Positive control

    • Recombinant Human TICAM2 protein (ab123160) can be used as a positive control in WB. This antibody gave a positive signal in Human Kidney and Human Spleen Tissue Lyates
  • General notes

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.02% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • Purity

    IgG fraction
  • Clonality

    Monoclonal
  • Clone number

    mAbcam77169
  • Myeloma

    Sp2/0-Ag14
  • Isotype

    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab77169 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 10 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 27 kDa).
Flow Cyt Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Functions in LPS-TLR4 signaling to regulate the MYD88-independent pathway during the innate immune response to LPS. Also involved in IL1-triggered NF-kappa-B activation, functioning upstream of IRAK1, IRAK2, TRAF6, and IKBKB. Physically bridges TLR4 and TICAM1 and functionally transmits LPS-TRL4 signal to TICAM1.
  • Tissue specificity

    Expressed in spleen, prostate, testis, uterus, small intestine, colon, peripheral blood leukocytes, heart, placenta, lung, liver, skeletal muscle, and pancreas.
  • Sequence similarities

    Contains 1 TIR domain.
  • Domain

    The TIR domain mediates the interaction with TRAF6.
  • Post-translational
    modifications

    Phosphorylated by PKCE in response to LPS. Phosphorylation is essential for its function. It is depleted from the membrane upon phosphorylation.
    Myristoylated. Required for membrane association which is critical for its ability to initiate efficient signaling.
  • Cellular localization

    Cytoplasm. Golgi apparatus. Cell membrane. Localized to the plasma membrane as a result of myristoylation. Phosphorylation on Ser-16 leads to its depletion from the membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Putative NF-kappa-B-activating protein 502 antibody
    • TCAM2_HUMAN antibody
    • TICAM-2 antibody
    • TICAM2 antibody
    • TIR containing adapter molecule 2 antibody
    • TIR domain containing adaptor protein antibody
    • TIR domain-containing adapter molecule 2 antibody
    • TIRAP 3 antibody
    • TIRAP3 antibody
    • TIRAP3a short form antibody
    • TIRP antibody
    • Toll-like receptor adaptor protein 3 antibody
    • Toll/interleukin-1 receptor domain-containing protein antibody
    • TRAM antibody
    • TRIF-related adapter molecule antibody
    see all

Images

  • All lanes : Anti-TICAM2 antibody [mAbcam77169] (ab77169) at 10 µg/ml

    Lane 1 : Human kidney tissue lysate - total protein (ab30203)
    Lane 2 : Human spleen tissue lysate - total protein (ab29699)

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 27 kDa
    Observed band size: 32 kDa
    why is the actual band size different from the predicted?

  • Overlay histogram showing PC3 cells stained with ab77169 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab77169, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References

ab77169 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab77169.
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