Recombinant Anti-TID1 antibody [EPR12414] (ab181024)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12414] to TID1
- Suitable for: WB, ICC/IF, IP
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-TID1 antibody [EPR12414]
See all TID1 primary antibodies -
Description
Rabbit monoclonal [EPR12414] to TID1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- JAR, Jurkat, HeLa and K562 cell lysate. HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR12414 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab181024 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 52 kDa.
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ICC/IF |
1/100 - 1/250.
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IP |
1/30.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 52 kDa. |
ICC/IF
1/100 - 1/250. |
IP
1/30. |
Target
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Relevance
TID1 is a human homolog of the Drosophila tumor suppressor lethal tumerous imaginal discs and encodes two mitochondrial matrix localized splice variants of human Tid1 designated hTid1S and hTid1L. These proteins are the conserved members of the DnaJ family of proteins which act as cochaperons for mitochondrial Hsp70. They contain a conserved tetrahedrical J domain which binds to Hsp70 chaperones and activates their ATPase activity. Expression of hTid1L increases apoptosis induced by DNA damaging agents as mitomycin C and TNF alpha. A J domain mutant of hTid1L can dominantly suppress apoptosis and in sharp contrast the J domain mutant of hTid1S increases apoptosis. Expression of hTid1S and hTid1L affects cytochrome c release from the mitochondria and caspase 3 activation, while activation of caspase 8 is unaffected. It is strongly suggested that these two splice variants exert their anti and pro apoptotic effects through discrete substrates and activities. Hence the relative abundance of these proteins or their substrates may allow the mitochondria to dampen or enhance the apoptotic signals. -
Cellular localization
Mitochondrial -
Database links
- Entrez Gene: 9093 Human
- Entrez Gene: 83945 Mouse
- Entrez Gene: 360481 Rat
- Omim: 608382 Human
- SwissProt: Q96EY1 Human
- SwissProt: Q99M87 Mouse
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Alternative names
- DnaJ (Hsp40) homolog subfamily A member 3 antibody
- DnaJ homolog subfamily A member 3 mitochondrial precursor antibody
- DnaJ protein Tid 1 antibody
see all
Images
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All lanes : Anti-TID1 antibody [EPR12414] (ab181024) at 1/5000 dilution
Lane 1 : JAR cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : K562 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 52 kDaBlocking/ Dilution buffer: 5% NFDM /TBST.
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Immunofluorescent analysis of acetone fixed HeLa cells labeling TID1 using ab181024 at a 1/250 dilution. A Goat anti rabbit IgG (Alexa Fluor®555) was used as the secondary at a 1/200 dilution. Counterstain DAPI.
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Lysate from Jurkat cells (Lane 1) and negative control (Lane 2) were immunoprecipitated with ab181024 at a 1/30 dilution. A specific to the non-reduced form of IgG at a 1/1500 dilution for the secondary. Blocking/ Dilution buffer: 5% NFDM/TBST.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (1)
ab181024 has been referenced in 1 publication.
- Konovalova S et al. Exposure to arginine analog canavanine induces aberrant mitochondrial translation products, mitoribosome stalling, and instability of the mitochondrial proteome. Int J Biochem Cell Biol 65:268-74 (2015). WB ; Human . PubMed: 26117454