Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12414] to TID1
- Suitable for: WB, ICC/IF, IP
- Reacts with: Mouse, Rat, Human
Product nameAnti-TID1 antibody [EPR12414]
See all TID1 primary antibodies
DescriptionRabbit monoclonal [EPR12414] to TID1
Tested applicationsSuitable for: WB, ICC/IF, IPmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human TID1 aa 450 to the C-terminus. The exact sequence is proprietary.
Database link: Q96EY1
- JAR, Jurkat, HeLa and K562 cell lysate. HeLa cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab181024 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 52 kDa.|
|ICC/IF||1/100 - 1/250.|
RelevanceTID1 is a human homolog of the Drosophila tumor suppressor lethal tumerous imaginal discs and encodes two mitochondrial matrix localized splice variants of human Tid1 designated hTid1S and hTid1L. These proteins are the conserved members of the DnaJ family of proteins which act as cochaperons for mitochondrial Hsp70. They contain a conserved tetrahedrical J domain which binds to Hsp70 chaperones and activates their ATPase activity. Expression of hTid1L increases apoptosis induced by DNA damaging agents as mitomycin C and TNF alpha. A J domain mutant of hTid1L can dominantly suppress apoptosis and in sharp contrast the J domain mutant of hTid1S increases apoptosis. Expression of hTid1S and hTid1L affects cytochrome c release from the mitochondria and caspase 3 activation, while activation of caspase 8 is unaffected. It is strongly suggested that these two splice variants exert their anti and pro apoptotic effects through discrete substrates and activities. Hence the relative abundance of these proteins or their substrates may allow the mitochondria to dampen or enhance the apoptotic signals.
- DnaJ (Hsp40) homolog subfamily A member 3 antibody
- DnaJ homolog subfamily A member 3 mitochondrial precursor antibody
- DnaJ protein Tid 1 antibody
All lanes : Anti-TID1 antibody [EPR12414] (ab181024) at 1/5000 dilution
Lane 1 : JAR cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : K562 cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 52 kDa
Blocking/ Dilution buffer: 5% NFDM /TBST.
Immunofluorescent analysis of acetone fixed HeLa cells labeling TID1 using ab181024 at a 1/250 dilution. A Goat anti rabbit IgG (Alexa Fluor®555) was used as the secondary at a 1/200 dilution. Counterstain DAPI.
Lysate from Jurkat cells (Lane 1) and negative control (Lane 2) were immunoprecipitated with ab181024 at a 1/30 dilution. A specific to the non-reduced form of IgG at a 1/1500 dilution for the secondary. Blocking/ Dilution buffer: 5% NFDM/TBST.
ab181024 has been referenced in 1 publication.
- Konovalova S et al. Exposure to arginine analog canavanine induces aberrant mitochondrial translation products, mitoribosome stalling, and instability of the mitochondrial proteome. Int J Biochem Cell Biol 65:268-74 (2015). WB ; Human . PubMed: 26117454