Anti-TIE1 antibody [8C9] (ab27851)

Product name

Anti-TIE1 antibody [8C9]
See all TIE1 primary antibodies
Description

Mouse monoclonal [8C9] to TIE1
Host species

Mouse
Tested applications

Suitable for: ELISA, WB, Flow Cytmore details
Species reactivity

Reacts with: Human
Immunogen

Recombinant fragment corresponding to Human TIE1. Recombinant human soluble extracellular TIE1.
Database link: P35590

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: None
Constituents: PBS, pH 7.4.
Concentration information loading...
Purity

Protein G purified
Purification notes

This antibody was purified from cell culture supernatant by chromatography.
Clonality

Monoclonal
Clone number

8C9
Isotype

IgG1
Research areas

Our Abpromise guarantee covers the use of ab27851 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
ELISA		Use a concentration of 1 - 10 µg/ml.
WB		Use a concentration of 200000 - 500000 µg/ml. Predicted molecular weight: 125 kDa.
Flow Cyt		Use 1µg for 10⁶ cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Application

Abreviews

Notes

ELISA

Use a concentration of 1 - 10 µg/ml.

WB

Use a concentration of 200000 - 500000 µg/ml. Predicted molecular weight: 125 kDa.

Flow Cyt

Use 1µg for 10⁶ cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Function

Transmembrane tyrosine-protein kinase that may modulate TEK/TIE2 activity and contribute to the regulation of angiogenesis.
Tissue specificity

Specifically expressed in developing vascular endothelial cells.
Sequence similarities

Belongs to the protein kinase superfamily. Tyr protein kinase family. Tie subfamily.
Contains 3 EGF-like domains.
Contains 3 fibronectin type-III domains.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain.
Post-translational
modifications

Phosphorylated on tyrosine residues in response to ANGPT1, most likely by TEK/TIE2.
Cellular localization

Cell membrane.
Target information above from: UniProt accession P35590 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 7075 Human
- Omim: 600222 Human
- SwissProt: P35590 Human
- Unigene: 78824 Human
Alternative names
- JKT 14 antibody
- JTK14 antibody
- TIE antibody
- Tie1 antibody
- TIE1_HUMAN antibody
- Tyrosine kinase with immunoglobulin and epidermal growth factor homology domains 1 antibody
- Tyrosine Kinase with Immunoglobulin and Epidermal Growth Factor Homology Domains antibody
- Tyrosine kinase with immunoglobulin-like and EGF-like domains 1 antibody
- Tyrosine protein kinase receptor Tie 1 antibody
- Tyrosine-protein kinase receptor Tie-1 antibody
see all

Flow Cytometry - Anti-TIE1 antibody [8C9] (ab27851)

Overlay histogram showing K562 cells stained with ab27851 (red line). The cells were fixed with 80% methanol (5 min) and then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab27851, 1µg/1x10⁶ cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x10⁶ cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in K562 cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.
Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
Western blot - Anti-TIE1 antibody [8C9] (ab27851)

All lanes : Anti-TIE1 antibody [8C9] (ab27851)

Lane 1 : Recombinant human soluble TIE1
Lane 2 : Recombinant human soluble TIE2
Lane 3 : Recombinant mouse soluble TIE1
Lane 4 : Recombinant mouse soluble TIE2

Predicted band size: 125 kDa

Lipps C et al. Expansion of functional personalized cells with specific transgene combinations. Nat Commun 9:994 (2018). Read more (PubMed: 29520052) »

Publishing research using ab27851? Please let us know so that we can cite the reference in this datasheet.

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