Anti-TIE1 antibody [8C9] (ab27851)

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Flow Cytometry - Anti-TIE1 antibody [8C9] (ab27851)
  • Western blot - Anti-TIE1 antibody [8C9] (ab27851)

Key features and details

  • Mouse monoclonal [8C9] to TIE1
  • Suitable for: WB, Flow Cyt
  • Reacts with: Human, Recombinant fragment
  • Isotype: IgG1

Overview

  • Product name

    Anti-TIE1 antibody [8C9]
    See all TIE1 primary antibodies

  • Description

    Mouse monoclonal [8C9] to TIE1

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, Flow Cytmore details

  • Species reactivity

    Reacts with: Human, Recombinant fragment

  • Immunogen

    Recombinant fragment corresponding to Human TIE1. Recombinant human soluble extracellular TIE1.
    Database link: P35590

  • Positive control

    • Flow Cyt: K562 (Human chronic myelogenous leukemia lymphoblast cell line ) whole cell lysate WB: Recombinant human soluble TIE1

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab27851 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use a concentration of 200000 - 500000 µg/ml. Predicted molecular weight: 125 kDa.
Flow Cyt
Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 
Notes
WB
Use a concentration of 200000 - 500000 µg/ml. Predicted molecular weight: 125 kDa.
Flow Cyt
Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Transmembrane tyrosine-protein kinase that may modulate TEK/TIE2 activity and contribute to the regulation of angiogenesis.

  • Tissue specificity

    Specifically expressed in developing vascular endothelial cells.

  • Sequence similarities

    Belongs to the protein kinase superfamily. Tyr protein kinase family. Tie subfamily.
    Contains 3 EGF-like domains.
    Contains 3 fibronectin type-III domains.
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.

  • Post-translational
    modifications

    Phosphorylated on tyrosine residues in response to ANGPT1, most likely by TEK/TIE2.

  • Cellular localization

    Cell membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • JKT 14 antibody
      • JTK14 antibody
      • TIE antibody
      • Tie1 antibody
      • TIE1_HUMAN antibody
      • Tyrosine kinase with immunoglobulin and epidermal growth factor homology domains 1 antibody
      • Tyrosine Kinase with Immunoglobulin and Epidermal Growth Factor Homology Domains antibody
      • Tyrosine kinase with immunoglobulin-like and EGF-like domains 1 antibody
      • Tyrosine protein kinase receptor Tie 1 antibody
      • Tyrosine-protein kinase receptor Tie-1 antibody
      see all

    Images

    • Flow Cytometry - Anti-TIE1 antibody [8C9] (ab27851)
      Flow Cytometry - Anti-TIE1 antibody [8C9] (ab27851)
      Overlay histogram showing K562 cells stained with ab27851 (red line). The cells were fixed with 80% methanol (5 min) and then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab27851, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in K562 cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.
      Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
    • Western blot - Anti-TIE1 antibody [8C9] (ab27851)
      Western blot - Anti-TIE1 antibody [8C9] (ab27851)
      All lanes : Anti-TIE1 antibody [8C9] (ab27851)

      Lane 1 : Recombinant human soluble TIE1
      Lane 2 : Recombinant human soluble TIE2
      Lane 3 : Recombinant mouse soluble TIE1
      Lane 4 : Recombinant mouse soluble TIE2

      Predicted band size: 125 kDa

    References (2)

    Publishing research using ab27851? Please let us know so that we can cite the reference in this datasheet.

    ab27851 has been referenced in 2 publications.

    • Zhang J  et al. Neurotrophin-3 acts on the endothelial-mesenchymal transition of heterotopic ossification in rats. J Cell Mol Med 23:2595-2609 (2019). PubMed: 30672120
    • Lipps C  et al. Expansion of functional personalized cells with specific transgene combinations. Nat Commun 9:994 (2018). PubMed: 29520052

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