Key features and details
- Mouse monoclonal [8C9] to TIE1
- Suitable for: ELISA, WB, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-TIE1 antibody [8C9]
See all TIE1 primary antibodies
DescriptionMouse monoclonal [8C9] to TIE1
Tested applicationsSuitable for: ELISA, WB, Flow Cytmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human TIE1. Recombinant human soluble extracellular TIE1.
Database link: P35590
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Concentration information loading...
PurityProtein G purified
Purification notesThis antibody was purified from cell culture supernatant by chromatography.
Our Abpromise guarantee covers the use of ab27851 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use a concentration of 1 - 10 µg/ml.|
|WB||Use a concentration of 200000 - 500000 µg/ml. Predicted molecular weight: 125 kDa.|
|Flow Cyt||Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
FunctionTransmembrane tyrosine-protein kinase that may modulate TEK/TIE2 activity and contribute to the regulation of angiogenesis.
Tissue specificitySpecifically expressed in developing vascular endothelial cells.
Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. Tie subfamily.
Contains 3 EGF-like domains.
Contains 3 fibronectin type-III domains.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain.
modificationsPhosphorylated on tyrosine residues in response to ANGPT1, most likely by TEK/TIE2.
Cellular localizationCell membrane.
- Information by UniProt
- JKT 14 antibody
- JTK14 antibody
- TIE antibody
Overlay histogram showing K562 cells stained with ab27851 (red line). The cells were fixed with 80% methanol (5 min) and then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab27851, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in K562 cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.
Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
All lanes : Anti-TIE1 antibody [8C9] (ab27851)
Lane 1 : Recombinant human soluble TIE1
Lane 2 : Recombinant human soluble TIE2
Lane 3 : Recombinant mouse soluble TIE1
Lane 4 : Recombinant mouse soluble TIE2
Predicted band size: 125 kDa
ab27851 has been referenced in 2 publications.
- Zhang J et al. Neurotrophin-3 acts on the endothelial-mesenchymal transition of heterotopic ossification in rats. J Cell Mol Med 23:2595-2609 (2019). PubMed: 30672120
- Lipps C et al. Expansion of functional personalized cells with specific transgene combinations. Nat Commun 9:994 (2018). PubMed: 29520052