Anti-TIE1 antibody [8C9] (ab27851)
![Flow Cytometry - Anti-TIE1 antibody [8C9] (ab27851)](https://www.abcam.com/ps/products/27/ab27851/Images/ab27851-38793-anti-tie1-antibody-8c9-flow-cytometry.jpg "Flow Cytometry - Anti-TIE1 antibody [8C9] (ab27851)")
Key features and details
- Mouse monoclonal [8C9] to TIE1
- Suitable for: WB, Flow Cyt
- Reacts with: Human, Recombinant fragment
- Isotype: IgG1
Overview
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Product name
Anti-TIE1 antibody [8C9]
See all TIE1 primary antibodies -
Description
Mouse monoclonal [8C9] to TIE1 -
Host species
Mouse -
Tested applications
Suitable for: WB, Flow Cytmore details -
Species reactivity
Reacts with: Human, Recombinant fragment -
Immunogen
Recombinant fragment corresponding to Human TIE1. Recombinant human soluble extracellular TIE1.
Database link: P35590 -
Positive control
- Flow Cyt: K562 (Human chronic myelogenous leukemia lymphoblast cell line ) whole cell lysate WB: Recombinant human soluble TIE1
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
This antibody was purified from cell culture supernatant by chromatography. -
Clonality
Monoclonal -
Clone number
8C9 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab27851 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 200000 - 500000 µg/ml. Predicted molecular weight: 125 kDa.
|
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| Flow Cyt |
Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|
| Notes |
|---|
|
WB
Use a concentration of 200000 - 500000 µg/ml. Predicted molecular weight: 125 kDa. |
|
Flow Cyt
Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|
Target
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Function
Transmembrane tyrosine-protein kinase that may modulate TEK/TIE2 activity and contribute to the regulation of angiogenesis. -
Tissue specificity
Specifically expressed in developing vascular endothelial cells. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. Tie subfamily.
Contains 3 EGF-like domains.
Contains 3 fibronectin type-III domains.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain. -
Post-translational
modificationsPhosphorylated on tyrosine residues in response to ANGPT1, most likely by TEK/TIE2. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 7075 Human
- Omim: 600222 Human
- SwissProt: P35590 Human
- Unigene: 78824 Human
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Alternative names
- JKT 14 antibody
- JTK14 antibody
- TIE antibody
see all
Images
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Flow Cytometry - Anti-TIE1 antibody [8C9] (ab27851)Overlay histogram showing K562 cells stained with ab27851 (red line). The cells were fixed with 80% methanol (5 min) and then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab27851, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in K562 cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.
Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback. -
![Western blot - Anti-TIE1 antibody [8C9] (ab27851)](https://www.abcam.com/ps/products/27/ab27851/Images/ab27851-232684-anti-tie1-antibody-8c9-western-blot.jpg)
Western blot - Anti-TIE1 antibody [8C9] (ab27851)All lanes : Anti-TIE1 antibody [8C9] (ab27851)
Lane 1 : Recombinant human soluble TIE1
Lane 2 : Recombinant human soluble TIE2
Lane 3 : Recombinant mouse soluble TIE1
Lane 4 : Recombinant mouse soluble TIE2
Predicted band size: 125 kDa
Datasheets and documents
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Datasheet download
References (4)
ab27851 has been referenced in 4 publications.
- Zhang J et al. Melatonin Promotes Heterotopic Ossification Through Regulation of Endothelial-Mesenchymal Transition in Injured Achilles Tendons in Rats. Front Cell Dev Biol 9:629274 (2021). PubMed: 33644068
- Liu T et al. MiR-4729 regulates TIE1 mRNA m6A modification and angiogenesis in hemorrhoids by targeting METTL14. Ann Transl Med 9:232 (2021). PubMed: 33708859
- Zhang J et al. Neurotrophin-3 acts on the endothelial-mesenchymal transition of heterotopic ossification in rats. J Cell Mol Med 23:2595-2609 (2019). PubMed: 30672120
- Lipps C et al. Expansion of functional personalized cells with specific transgene combinations. Nat Commun 9:994 (2018). PubMed: 29520052
