Anti-TIE1 antibody [8C9] (ab27851)
- Datasheet
- Specific References (1)
- Protocols
Overview
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Product nameAnti-TIE1 antibody [8C9]
See all TIE1 primary antibodies -
DescriptionMouse monoclonal [8C9] to TIE1
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Host speciesMouse
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Tested applicationsSuitable for: ELISA, WB, Flow Cytmore details
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Species reactivityReacts with: Human
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Immunogen
Recombinant fragment corresponding to Human TIE1. Recombinant human soluble extracellular TIE1.
Database link: P35590
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage bufferPreservative: None
Constituents: PBS, pH 7.4. -
Concentration information loading... -
PurityProtein G purified
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Purification notesThis antibody was purified from cell culture supernatant by chromatography.
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ClonalityMonoclonal
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Clone number8C9
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IsotypeIgG1
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab27851 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA | Use a concentration of 1 - 10 µg/ml. | |
| WB | Use a concentration of 200000 - 500000 µg/ml. Predicted molecular weight: 125 kDa. | |
| Flow Cyt | Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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Target
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FunctionTransmembrane tyrosine-protein kinase that may modulate TEK/TIE2 activity and contribute to the regulation of angiogenesis.
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Tissue specificitySpecifically expressed in developing vascular endothelial cells.
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Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. Tie subfamily.
Contains 3 EGF-like domains.
Contains 3 fibronectin type-III domains.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain. -
Post-translational
modificationsPhosphorylated on tyrosine residues in response to ANGPT1, most likely by TEK/TIE2. -
Cellular localizationCell membrane.
- Information by UniProt
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Database links
- Entrez Gene: 7075 Human
- Omim: 600222 Human
- SwissProt: P35590 Human
- Unigene: 78824 Human
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Alternative names
- JKT 14 antibody
- JTK14 antibody
- TIE antibody
see all
Images
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![Flow Cytometry - Anti-TIE1 antibody [8C9] (ab27851)](https://www.abcam.com/ps/products/27/ab27851/Images/TIE1-Primary-antibodies-ab27851-1.jpg)
Flow Cytometry - Anti-TIE1 antibody [8C9] (ab27851)Overlay histogram showing K562 cells stained with ab27851 (red line). The cells were fixed with 80% methanol (5 min) and then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab27851, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in K562 cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.
Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback. -
![Western blot - Anti-TIE1 antibody [8C9] (ab27851)](https://www.abcam.com/ps/products/27/ab27851/Images/ab27851-232684-ab27851wb.jpg)
Western blot - Anti-TIE1 antibody [8C9] (ab27851)All lanes : Anti-TIE1 antibody [8C9] (ab27851)
Lane 1 : Recombinant human soluble TIE1
Lane 2 : Recombinant human soluble TIE2
Lane 3 : Recombinant mouse soluble TIE1
Lane 4 : Recombinant mouse soluble TIE2
Predicted band size: 125 kDa
References
This product has been referenced in:
- Lipps C et al. Expansion of functional personalized cells with specific transgene combinations. Nat Commun 9:994 (2018). Read more (PubMed: 29520052) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"