Recombinant
RabMAb

Recombinant Anti-TIE2 + TIE1 (phospho Y992 + Y1007) antibody [EPR1053(N)(B)] - BSA and Azide free (ab229195)

Overview

  • Product name

    Anti-TIE2 + TIE1 (phospho Y992 + Y1007) antibody [EPR1053(N)(B)] - BSA and Azide free
    See all TIE2 + TIE1 primary antibodies
  • Description

    Rabbit monoclonal [EPR1053(N)(B)] to TIE2 + TIE1 (phospho Y992 + Y1007) - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, Dot blot, WBmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human TIE2 + TIE1 (phospho Y992). The exact sequence is proprietary.

  • General notes

    ab229195 is the carrier-free version of ab151704 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab229195 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR1053(N)(B)
  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab229195 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
Dot blot Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 125 kDa.
  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Database links

    • Alternative names

      • Angiopoietin-1 receptor antibody
      • CD202b antibody
      • CD202b antigen antibody
      • Endothelial tyrosine kinase antibody
      • Endothelium specific receptor tyrosine kinase 2 antibody
      • hTIE 2 antibody
      • hTIE2 antibody
      • Hyk antibody
      • p140 TEK antibody
      • TEK antibody
      • tek tyrosine kinase antibody
      • TEK tyrosine kinase endothelial antibody
      • TIE 1 antibody
      • TIE 2 antibody
      • TIE antibody
      • Tunica interna endothelial cell kinase antibody
      • Tyrosine kinase with Ig and EGF homology domains-2 antibody
      • Tyrosine-protein kinase receptor TEK antibody
      • Tyrosine-protein kinase receptor Tie-1 antibody
      • Tyrosine-protein kinase receptor TIE-2 antibody
      • VMCM 1 antibody
      • VMCM antibody
      • VMCM1 antibody
      see all

    Images

    • Immunocytochemistry/Immunofluorescence analysis of HUVEC () cells labelling TIE2 + TIE1 (phospho Y992 + Y1007) with ab151704 at 1/500. Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton-X. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Cells were counter-stained with ab7291 anti-Tubulin (mouse mAb) at a dilution of 1/500 and ab150120 AlexaFluor®594 Goat anti-Mouse secondary at 1/1000. Nuclei were counterstained with DAPI (blue).

      Confocal image showing increased cytoplasmic staining after PER (Pervanadate, 1mM, 30min) treatment on HUVEC cells. The LP treatment decreased the PER induced cytoplasmic staining. 

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab151704).

    • Dot blot analysis of TIE2 (pY992) phospho peptide (lane 1) and TIE2 non-phospho peptide (lane 2) labelling TIE2 (phospho Y992) with ab151704 at a dilution of 1/1000. A peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/2500).

      Blocking and dilution buffer: 5% NFDM/TBST.

      Exposure time: 10 seconds.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab151704).

    References

    ab229195 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab229195.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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