Anti-TRIM24 antibody (ab70560)
Key features and details
- Rabbit polyclonal to TRIM24
- Suitable for: IHC-P, IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-TRIM24 antibody
See all TRIM24 primary antibodies -
Description
Rabbit polyclonal to TRIM24 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, IP, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan -
Immunogen
Synthetic peptide within Human TRIM24 aa 700-750. The exact sequence is proprietary.
Database link: NP_056989.2 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab70560 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/200.
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IP |
Use at an assay dependent concentration.
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WB |
Use a concentration of 0.1 µg/ml. Detects a band of approximately 130 kDa (predicted molecular weight: 116 kDa).
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Notes |
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IHC-P
1/200. |
IP
Use at an assay dependent concentration. |
WB
Use a concentration of 0.1 µg/ml. Detects a band of approximately 130 kDa (predicted molecular weight: 116 kDa). |
Target
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Function
Transcriptional coactivator that interacts with numerous nuclear receptors and coactivators and modulates the transcription of target genes. Interacts with chromatin depending on histone H3 modifications, having the highest affinity for histone H3 that is both unmodified at 'Lys-4' (H3K4me0) and acetylated at 'Lys-23' (H3K23ac). Has E3 protein-ubiquitin ligase activity. Promotes ubiquitination and proteasomal degradation of p53/TP53. Plays a role in the regulation of cell proliferation and apoptosis, at least in part via its effects on p53/TP53 levels. Up-regulates ligand-dependent transcription activation by AR, GCR/NR3C1, thyroid hormone receptor (TR) and ESR1. Modulates transcription activation by retinoic acid (RA) receptors, including RARA. Plays a role in regulating retinoic acid-dependent proliferation of hepatocytes. -
Pathway
Protein modification; protein ubiquitination. -
Involvement in disease
Defects in TRIM24 are a cause of thyroid papillary carcinoma (TPC) [MIM:188550]. TPC is a common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue. Papillary carcinomas are malignant neoplasm characterized by the formation of numerous, irregular, finger-like projections of fibrous stroma that is covered with a surface layer of neoplastic epithelial cells. Note=A chromosomal aberration involving TRIM24/TIF1 is found in thyroid papillary carcinomas. Translocation t(7;10)(q32;q11) with RET. The translocation generates the TRIM24/RET (PTC6) oncogene. -
Sequence similarities
Contains 2 B box-type zinc fingers.
Contains 1 bromo domain.
Contains 1 PHD-type zinc finger.
Contains 1 RING-type zinc finger. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Sumoylated. -
Cellular localization
Nucleus. Cytoplasm. Colocalizes with sites of active transcription. Detected both in nucleus and cytoplasm in some breast cancer samples. Predominantly nuclear. - Information by UniProt
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Database links
- Entrez Gene: 8805 Human
- Entrez Gene: 21848 Mouse
- Entrez Gene: 500084 Rat
- Omim: 603406 Human
- SwissProt: O15164 Human
- SwissProt: Q64127 Mouse
- Unigene: 490287 Human
- Unigene: 41063 Mouse
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Alternative names
- E3 ubiquitin protein ligase TRIM24 antibody
- E3 ubiquitin-protein ligase Trim24 antibody
- hTIF1 antibody
see all
Images
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All lanes : Anti-TRIM24 antibody (ab70560) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : HEK293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : TCMK-1 whole cell lysate
Lane 5 : NIH 3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 116 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse teratoma tissue labelling TRIM24 with ab70560 at 1/200. Detection: DAB.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling TRIM24 with ab70560 at 1/200. Detection: DAB.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab70560 has been referenced in 7 publications.
- Xiao M et al. Long non-coding RNA ZFPM2-AS1 promotes colorectal cancer progression by sponging miR-137 to regulate TRIM24. Mol Med Rep 23:N/A (2021). PubMed: 33300060
- Mojsa B et al. Identification of SUMO Targets Associated With the Pluripotent State in Human Stem Cells. Mol Cell Proteomics 20:100164 (2021). PubMed: 34673284
- Chen M et al. NCK1-AS1 Increases Drug Resistance of Glioma Cells to Temozolomide by Modulating miR-137/TRIM24. Cancer Biother Radiopharm 35:101-108 (2020). PubMed: 31750728
- Xie H et al. Low let-7d exosomes from pulmonary vascular endothelial cells drive lung pericyte fibrosis through the TGFßRI/FoxM1/Smad/ß-catenin pathway. J Cell Mol Med 24:13913-13926 (2020). PubMed: 33179861
- Fyfe JJ et al. Enhanced skeletal muscle ribosome biogenesis, yet attenuated mTORC1 and ribosome biogenesis-related signalling, following short-term concurrent versus single-mode resistance training. Sci Rep 8:560 (2018). PubMed: 29330460
- Fang Z et al. TRIM24 promotes the aggression of gastric cancer via the Wnt/ß-catenin signaling pathway. Oncol Lett 13:1797-1806 (2017). WB, IHC-P ; Human . PubMed: 28454326
- Fang Z et al. Regulation of TRIM24 by miR-511 modulates cell proliferation in gastric cancer. J Exp Clin Cancer Res 36:17 (2017). WB . PubMed: 28114950