Recombinant
RabMAb

Recombinant Anti-TIFA (phospho T9) antibody [EPR19853] - BSA and Azide free (ab236848)

Overview

  • Product name

    Anti-TIFA (phospho T9) antibody [EPR19853] - BSA and Azide free
    See all TIFA primary antibodies
  • Description

    Rabbit monoclonal [EPR19853] to TIFA (phospho T9) - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, Dot blot, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human TIFA aa 1-100. The exact sequence is proprietary.
    Database link: Q96CG3

  • Positive control

    • WB: HeLa transfected with 3×Flag-tagged TIFA expression vector for 24 hours, and then infected with S. flexneri 2457T for 4 hours, whole cell lysate.
  • General notes

    Ab236848 is the carrier-free version of ab214815. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab236848 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

Properties

Applications

Our Abpromise guarantee covers the use of ab236848 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
Dot blot Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 21 kDa.

Target

  • Function

    Adapter protein which mediates the IRAK1 and TRAF6 interaction following IL-1 stimulation, resulting in the downstream activation of NF-kappa-B and AP-1 pathways. Induces the oligomerization and polyubiquitination of TRAF6, which leads to the activation of TAK1 and IKK through a proteasome-independent mechanism.
  • Sequence similarities

    Contains 1 FHA domain.
  • Information by UniProt
  • Database links

  • Alternative names

    • Putative MAPK activating protein PM14 antibody
    • Putative MAPK-activating protein PM14 antibody
    • Putative NF kappa B activating protein 20 antibody
    • Putative NF-kappa-B-activating protein 20 antibody
    • T2BP antibody
    • TIFA antibody
    • TIFA_HUMAN antibody
    • TRAF interacting protein with FHA domain containing protein A antibody
    • TRAF-interacting protein with FHA domain-containing protein A antibody
    • TRAF2 binding protein antibody
    • TRAF2-binding protein antibody
    see all

Images

  • All lanes : Anti-TIFA (phospho T9) antibody [EPR19853] (ab214815) at 1/1000 dilution

    Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) transfected with 3×Flag-tagged TIFA expression vector for 28 hours, whole cell lysate
    Lane 2 : HeLa transfected with 3×Flag-tagged TIFA T9A mutant expression vector for 28 hours, whole cell lysate
    Lane 3 : HeLa transfected with 3×Flag-tagged TIFA expression vector for 24 hours, and then infected with S. flexneri 2457T for 4 hours, whole cell lysate
    Lane 4 : HeLa transfected with 3×Flag-tagged TIFAT9A mutant expression vector for 24 hours, and then infected with S. flexneri 2457T for 4 hours, whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/5000 dilution

    Predicted band size: 21 kDa


    Exposure time: 30 seconds


    Blocking/Dilution buffer: 5% milk/TBST.

    The image was kindly provided by our collaborator Dr. Feng Shao, NIBS.

     Sflexneri infection induces an innate immune response (PMID: 28222186).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab214815).

  • TIFA (phospho T9) was immunoprecipitated from 0.35 mg HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate with ab214815 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab214815 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

    Lane 1: HEK-293T transfected with GFP-tagged TIFA expression vector for 24 hours, and then infected with B. cenocepacia J2315 for 4 hours whole cell lysate 10 µg (Input).
    Lane 2: ab214815 IP in HEK-293T transfected with GFP-tagged TIFA expression vector for 24 hours, and then infected with B. cenocepacia J2315 for 4 hours whole cell lysate (+).
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab214815 in HEK-293T transfected with GFP-tagged TIFA expression vector for 24 hours, and then infected with B. cenocepacia J2315 for 4 hours whole cell lysate (-).

    Blocking/Dilution buffer: 5% NFDM/TBST.
    Exposure time: 3 minutes.

    The cell lysates were kindly provided by our collaborator Dr. Feng Shao, NIBS.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab214815).

  • Dot blot analysis of TIFA (phospho T9) labeled with ab214815 at 1/1000 dilution.

    Lane 1: TIFA (phospho T9) peptide (aa3-12).

    Lane 2: TIFA (phospho T9) peptide (aa5-15).

    Lane 3: TIFA non-phospho peptide (aa3-15).

    Secondary used was Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: 10 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab214815).

References

ab236848 has not yet been referenced specifically in any publications.

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