Overview

  • Product name

  • Description

    Rabbit polyclonal to TIGAR
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human TIGAR (internal sequence). 19 amino acid peptide.
    Database link: Q9NQ88

  • Positive control

    • Human breast tissue; Human brain cells; MCF7 cell lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab189038 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 20 µg/ml.
WB Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 30 kDa.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Probable fructose-biphosphatase. Lowers cellular levels of fructose 2,6-bisphosphate. Protects cells against reactive oxygen species and against apoptosis induced by p53/TP53.
  • Sequence similarities

    Belongs to the phosphoglycerate mutase family.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Information by UniProt
  • Database links

  • Alternative names

    • 6-bisphosphatase TIGAR antibody
    • C12ORF5 antibody
    • chromosome 12 open reading frame 5 antibody
    • FR2BP antibody
    • Fructose-2,6-bisphosphatase TIGAR antibody
    • Fructose-2,6-bisphosphate 2-phosphatase antibody
    • Probable fructose 2,6 bisphosphatase TIGAR antibody
    • Probable fructose-2 antibody
    • tigar antibody
    • TIGAR_HUMAN antibody
    • TP53 induced glycolysis and apoptosis regulator antibody
    • TP53 induced glycolysis regulatory phosphatase antibody
    • TP53-induced glycolysis and apoptosis regulator antibody
    • Transactivated by NS3TP2 protein antibody
    see all

Images

  • Lane 1 : Anti-TIGAR antibody (ab189038) at 0.5 µg/ml
    Lane 2 : Anti-TIGAR antibody (ab189038) at 1 µg/ml
    Lane 3 : Anti-TIGAR antibody (ab189038) at 2 µg/ml

    All lanes : MCF7 cell lysate

    Predicted band size: 30 kDa

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human breast tissue labeling TIGAR with ab189038 at 5 µg/ml, followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

  • Immunofluorescent analysis of Human brain cells labeling TIGAR with ab189038 at 20 µg/ml.

References

ab189038 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab189038.
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