Key features and details
- Rabbit polyclonal to TIGAR
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-TIGAR antibody
See all TIGAR primary antibodies
DescriptionRabbit polyclonal to TIGAR
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide corresponding to Human TIGAR (internal sequence). 19 amino acid peptide.
Database link: Q9NQ88
- Human breast tissue; Human brain cells; MCF7 cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab189038 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 20 µg/ml.|
|WB||Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 30 kDa.|
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionProbable fructose-biphosphatase. Lowers cellular levels of fructose 2,6-bisphosphate. Protects cells against reactive oxygen species and against apoptosis induced by p53/TP53.
Sequence similaritiesBelongs to the phosphoglycerate mutase family.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- 6-bisphosphatase TIGAR antibody
- C12ORF5 antibody
- chromosome 12 open reading frame 5 antibody
Lane 1 : Anti-TIGAR antibody (ab189038) at 0.5 µg/ml
Lane 2 : Anti-TIGAR antibody (ab189038) at 1 µg/ml
Lane 3 : Anti-TIGAR antibody (ab189038) at 2 µg/ml
All lanes : MCF7 cell lysate
Predicted band size: 30 kDa
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human breast tissue labeling TIGAR with ab189038 at 5 µg/ml, followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Immunofluorescent analysis of Human brain cells labeling TIGAR with ab189038 at 20 µg/ml.
ab189038 has not yet been referenced specifically in any publications.