Key features and details
- Rabbit polyclonal to TIGAR
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-TIGAR antibody
See all TIGAR primary antibodies
DescriptionRabbit polyclonal to TIGAR
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Chimpanzee
- Human brain cerebellum tissue. Human, mouse and rat liver lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: 0.2% Gelatin, 99% PBS
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab189164 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 4 µg/ml. Predicted molecular weight: 30 kDa.|
|IHC-P||Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
FunctionProbable fructose-biphosphatase. Lowers cellular levels of fructose 2,6-bisphosphate. Protects cells against reactive oxygen species and against apoptosis induced by p53/TP53.
Sequence similaritiesBelongs to the phosphoglycerate mutase family.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- 6-bisphosphatase TIGAR antibody
- C12ORF5 antibody
- chromosome 12 open reading frame 5 antibody
Lanes 1-2 : Anti-TIGAR antibody (ab189164) at 1 µg/ml
Lane 3 : Anti-TIGAR antibody (ab189164) at 4 µg/ml
Lane 4 : Anti-TIGAR antibody (ab189164) at 2 µg/ml
Lane 1 : Human liver lysate
Lane 2 : Human liver lysate plus immunizing peptide
Lane 3 : Mouse liver lysate
Lane 4 : Rat liver lysate
Predicted band size: 30 kDa
Immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded human brain cerebellum tissue sections labeling TIGAR using ab189164 at 10 µg/ml.
ab189164 has been referenced in 1 publication.
- Li X et al. p53-TP53-Induced Glycolysis Regulator Mediated Glycolytic Suppression Attenuates DNA Damage and Genomic Instability in Fanconi Anemia Hematopoietic Stem Cells. Stem Cells 37:937-947 (2019). PubMed: 30977208