Overview

  • Product name

  • Description

    Rabbit polyclonal to TIGAR
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chimpanzee
  • Immunogen

    Synthetic peptide within Human TIGAR aa 140-190. The exact sequence is proprietary.
    Sequence:

    C QLILKEADQK EQFSQGSPSN CLETSLAEIF PLGKNHSSKV NSDSGIPGLA


    Database link: Q9NQ88

  • Positive control

    • Human brain cerebellum tissue. Human, mouse and rat liver lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituents: 0.2% Gelatin, 99% PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab189164 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 4 µg/ml. Predicted molecular weight: 30 kDa.
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Function

    Probable fructose-biphosphatase. Lowers cellular levels of fructose 2,6-bisphosphate. Protects cells against reactive oxygen species and against apoptosis induced by p53/TP53.
  • Sequence similarities

    Belongs to the phosphoglycerate mutase family.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Information by UniProt
  • Database links

  • Alternative names

    • 6-bisphosphatase TIGAR antibody
    • C12ORF5 antibody
    • chromosome 12 open reading frame 5 antibody
    • FR2BP antibody
    • Fructose-2,6-bisphosphatase TIGAR antibody
    • Fructose-2,6-bisphosphate 2-phosphatase antibody
    • Probable fructose 2,6 bisphosphatase TIGAR antibody
    • Probable fructose-2 antibody
    • tigar antibody
    • TIGAR_HUMAN antibody
    • TP53 induced glycolysis and apoptosis regulator antibody
    • TP53 induced glycolysis regulatory phosphatase antibody
    • TP53-induced glycolysis and apoptosis regulator antibody
    • Transactivated by NS3TP2 protein antibody
    see all

Images

  • Lanes 1-2 : Anti-TIGAR antibody (ab189164) at 1 µg/ml
    Lane 3 : Anti-TIGAR antibody (ab189164) at 4 µg/ml
    Lane 4 : Anti-TIGAR antibody (ab189164) at 2 µg/ml

    Lane 1 : Human liver lysate
    Lane 2 : Human liver lysate plus immunizing peptide
    Lane 3 : Mouse liver lysate
    Lane 4 : Rat liver lysate

    Predicted band size: 30 kDa

  • Immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded human brain cerebellum tissue sections labeling TIGAR using ab189164 at 10 µg/ml.

References

ab189164 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab189164.
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