Anti-TIGAR antibody (ab62533)
Key features and details
- Rabbit polyclonal to TIGAR
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-TIGAR antibody
See all TIGAR primary antibodies -
Description
Rabbit polyclonal to TIGAR -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IF, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide (Human) of 19 amino acids from a region near the centre of TIGAR
-
Positive control
- MCF7 cell lysate Human brain tissue
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab62533 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
Use a concentration of 2.5 µg/ml.
|
|
ICC/IF |
Use a concentration of 20 µg/ml.
|
|
WB |
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).
|
Notes |
---|
IHC-P
Use a concentration of 2.5 µg/ml. |
ICC/IF
Use a concentration of 20 µg/ml. |
WB
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa). |
Target
-
Function
Probable fructose-biphosphatase. Lowers cellular levels of fructose 2,6-bisphosphate. Protects cells against reactive oxygen species and against apoptosis induced by p53/TP53. -
Sequence similarities
Belongs to the phosphoglycerate mutase family. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. - Information by UniProt
-
Database links
- Entrez Gene: 57103 Human
- Omim: 610775 Human
- SwissProt: Q9NQ88 Human
- Unigene: 504545 Human
-
Alternative names
- 6-bisphosphatase TIGAR antibody
- C12ORF5 antibody
- chromosome 12 open reading frame 5 antibody
see all
Images
-
Lane 1 : Anti-TIGAR antibody (ab62533) at 0.5 µg/ml
Lane 2 : Anti-TIGAR antibody (ab62533) at 1 µg/ml
Lane 3 : Anti-TIGAR antibody (ab62533) at 2 µg/ml
All lanes : MCF7 cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 30 kDa
Observed band size: 30 kDa -
immunohistochemical analysis of TIGAR expression in human brain tissue using 2.5 µg/ml ab62533.
-
Immunofluorescence of TIGAR in Human Brain cells using ab62533 at 20 ug/ml.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (6)
ab62533 has been referenced in 6 publications.
- Chen F et al. TIGAR/AP-1 axis accelerates the division of Lgr5- reserve intestinal stem cells to reestablish intestinal architecture after lethal radiation. Cell Death Dis 11:501 (2020). PubMed: 32632140
- Roche ME et al. Translocase of the outer mitochondrial membrane complex subunit 20 (TOMM20) facilitates cancer aggressiveness and therapeutic resistance in chondrosarcoma. Biochim Biophys Acta Mol Basis Dis 1866:165962 (2020). PubMed: 32920118
- Wang H et al. Loss of TIGAR Induces Oxidative Stress and Meiotic Defects in Oocytes from Obese Mice. Mol Cell Proteomics 17:1354-1364 (2018). PubMed: 29776966
- Gooptu M et al. Mitochondrial and glycolytic metabolic compartmentalization in diffuse large B-cell lymphoma. Semin Oncol 44:204-217 (2017). PubMed: 29248132
- Maiti AK et al. IL-4 Protects the Mitochondria Against TNFa and IFN? Induced Insult During Clearance of Infection with Citrobacter rodentium and Escherichia coli. Sci Rep 5:15434 (2015). IHC ; Mouse . PubMed: 26481427
- Kim J et al. TIGAR regulates glycolysis in ischemic kidney proximal tubules. Am J Physiol Renal Physiol 308:F298-308 (2015). PubMed: 25503731