Overview

  • Product name

  • Description

    Rabbit polyclonal to TIGAR
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide (Human) of 19 amino acids from a region near the centre of TIGAR

  • Positive control

    • MCF7 cell lysate Human brain tissue

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab62533 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 2.5 µg/ml.
ICC/IF Use a concentration of 20 µg/ml.
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).

Target

  • Function

    Probable fructose-biphosphatase. Lowers cellular levels of fructose 2,6-bisphosphate. Protects cells against reactive oxygen species and against apoptosis induced by p53/TP53.
  • Sequence similarities

    Belongs to the phosphoglycerate mutase family.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Information by UniProt
  • Database links

  • Alternative names

    • 6-bisphosphatase TIGAR antibody
    • C12ORF5 antibody
    • chromosome 12 open reading frame 5 antibody
    • FR2BP antibody
    • Fructose-2,6-bisphosphatase TIGAR antibody
    • Fructose-2,6-bisphosphate 2-phosphatase antibody
    • Probable fructose 2,6 bisphosphatase TIGAR antibody
    • Probable fructose-2 antibody
    • tigar antibody
    • TIGAR_HUMAN antibody
    • TP53 induced glycolysis and apoptosis regulator antibody
    • TP53 induced glycolysis regulatory phosphatase antibody
    • TP53-induced glycolysis and apoptosis regulator antibody
    • Transactivated by NS3TP2 protein antibody
    see all

Images

  • Lane 1 : Anti-TIGAR antibody (ab62533) at 0.5 µg/ml
    Lane 2 : Anti-TIGAR antibody (ab62533) at 1 µg/ml
    Lane 3 : Anti-TIGAR antibody (ab62533) at 2 µg/ml

    All lanes : MCF7 cell lysate

    Lysates/proteins at 15 µg per lane.

    Predicted band size: 30 kDa
    Observed band size: 30 kDa

  • immunohistochemical analysis of TIGAR expression in human brain tissue using 2.5 µg/ml ab62533.
  • Immunofluorescence of TIGAR in Human Brain cells using ab62533 at 20 ug/ml.

References

This product has been referenced in:

  • Gooptu M  et al. Mitochondrial and glycolytic metabolic compartmentalization in diffuse large B-cell lymphoma. Semin Oncol 44:204-217 (2017). Read more (PubMed: 29248132) »
  • Maiti AK  et al. IL-4 Protects the Mitochondria Against TNFa and IFN? Induced Insult During Clearance of Infection with Citrobacter rodentium and Escherichia coli. Sci Rep 5:15434 (2015). IHC ; Mouse . Read more (PubMed: 26481427) »
See all 3 Publications for this product

Customer reviews and Q&As

Answer

Thank you for your inquiry.

Unfortunately, we cannot pinpoint exactly which of our antibodies was used for this publication.

With help of our records we can assume that it was ab62533

https://www.abcam.com/index.html?datasheet=62533 (or use the following: https://www.abcam.com/index.html?datasheet=62533).

I can suggest to ask the authors. They usually react favorably when contacted by other scientists.

I am sorry I could not be of more help and hope this information is nevertheless helpful.

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