Key features and details
- Mouse monoclonal [M2 - P4H2] to TIGAR
- Suitable for: WB, ELISA, Flow Cyt, ICC/IF
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-TIGAR antibody [M2 - P4H2]
See all TIGAR primary antibodies
DescriptionMouse monoclonal [M2 - P4H2] to TIGAR
Tested applicationsSuitable for: WB, ELISA, Flow Cyt, ICC/IFmore details
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human TIGAR aa 200-300.
- Recombinant Human TIGAR protein (ab115509) can be used as a positive control in WB. This antibody gave a positive signal in the following whole cell lysates: U20S cell line Jurkat cell line
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Concentration information loading...
Clone numberM2 - P4H2
Our Abpromise guarantee covers the use of ab64622 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 10 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).|
|ELISA||Use at an assay dependent concentration.|
|Flow Cyt||Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|ICC/IF||Use a concentration of 1 µg/ml.|
FunctionProbable fructose-biphosphatase. Lowers cellular levels of fructose 2,6-bisphosphate. Protects cells against reactive oxygen species and against apoptosis induced by p53/TP53.
Sequence similaritiesBelongs to the phosphoglycerate mutase family.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- 6-bisphosphatase TIGAR antibody
- C12ORF5 antibody
- chromosome 12 open reading frame 5 antibody
All lanes : Anti-TIGAR antibody [M2 - P4H2] (ab64622) at 10 µg/ml
Lane 1 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
Lane 2 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 30 kDa
Observed band size: 30 kDa
Additional bands at: 120 kDa, 37 kDa. We are unsure as to the identity of these extra bands.
ICC/IF image of ab64622 stained Hek293 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab64622, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HeLa, HepG2 and MCF7 cells fixed in 4% PFA at 1ug/ml.
Overlay histogram showing HeLa cells stained with ab64622 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab64622, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
ab64622 has been referenced in 1 publication.
- Xu X et al. Hypoxia-induced hsa-miR-101 promotes glycolysis by targeting TIGAR mRNA in clear cell renal cell carcinoma. Mol Med Rep 15:1373-1378 (2017). PubMed: 28138701