Recombinant Anti-TIGIT antibody [BLR047F] - BSA free (ab243903)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [BLR047F] to TIGIT - BSA free
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-TIGIT antibody [BLR047F] - BSA free
See all TIGIT primary antibodies -
Description
Rabbit monoclonal [BLR047F] to TIGIT - BSA free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human TIGIT aa 150 to the C-terminus (C terminal). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. NP_776160.2 and Gene ID 201633.
Database link: Q495A1 -
Positive control
- IHC-P: Human tonsil tissue
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General notes
This product is sold under License from Bethyl Laboratories, Inc.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.8
Preservative: 0.09% Sodium azide
Constituent: 99% Borate buffered saline -
Concentration information loading...
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Purification notes
Recombinant antibody was purified from cell culture supernatant. -
Clonality
Monoclonal -
Clone number
BLR047F -
Isotype
IgG -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab243903 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000.
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|
IHC-P | (5) |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
Notes |
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WB
1/1000. |
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Binds with high affinity to the poliovirus receptor (PVR) which causes increased secretion of IL10 and decreased secretion of IL12B and suppresses T cell activation by promoting the generation of mature immunoregulatory dendritic cells. -
Tissue specificity
Expressed at low levels on peripheral memory and regulatory CD4+ T cells and NK cells and is up-regulated following activation of these cells (at protein level). -
Sequence similarities
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Domain
Contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 201633 Human
- Omim: 612859 Human
- SwissProt: Q495A1 Human
- Unigene: 421750 Human
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Alternative names
- DKFZp667A205 antibody
- ENSMUSG00000071552 antibody
- FLJ39873 antibody
see all
Images
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Formalin-fixed, paraffin-embedded human tonsil tissue stained for TIGIT using ab243903 at 1/200 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DAB staining.
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Western blot analysis using ab243903 at 1/1000 dilution.
Lane 1: HeLa cell whole cell lysate (50 µg).
Lane 2: HEK-293T cell whole cell lysate (50 µg).
Lane 3: MyLa CD4+ whole cell lysate (50 µg).
Lane 4: HepG2 whole cell lysate (50 µg).
Lane 5: MG whole cell lysate (50 µg).
Lane 6: Jurkat whole cell lysate (50 µg).
Lane 7: GaMG whole cell lysate (50 µg).
Lane 8: A549 whole cell lysate (50 µg).
A HRP-conjugated goat anti-rabbit IgG antibody was used as the secondary. Detection: chemiluminescence with an exposure time of 3 minutes. -
Immunohistochemical analysis of Formalin/PFA-fixed paraffin-embedded human tonsil tissue sections labeling TIGIT with ab243903 (red). Dylight 594 conjugated goat-anti-rabbit IgG was used as secondary antibody. Counterstain: DAPI (blue).
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Immunohistochemical analysis of Formalin/PFA-fixed paraffin-embedded human tonsil tissue sections labeling TIGIT with ab243903 at 1/250 (red). HRP-conjugated goat anti-rabbit IgG was used as secondary antibody. Counterstain: DAPI (blue).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (16)
ab243903 has been referenced in 16 publications.
- Guo C et al. TIGIT as a Novel Prognostic Marker for Immune Infiltration in Invasive Breast Cancer. Comb Chem High Throughput Screen 26:639-651 (2023). PubMed: 35770416
- Hao B et al. Single-cell RNA sequencing analysis revealed cellular and molecular immune profiles in lung squamous cell carcinoma. Transl Oncol 27:101568 (2023). PubMed: 36270103
- Ma J et al. The immune checkpoint expression in the tumor immune microenvironment of DLBCL: Clinicopathologic features and prognosis. Front Oncol 12:1069378 (2022). PubMed: 36561512
- Nakazawa T et al. Expression of T-cell immunoreceptor with immunoglobulin and tyrosine-based inhibitory motif domains (TIGIT) in anaplastic thyroid carcinoma. BMC Endocr Disord 22:204 (2022). PubMed: 35971106
- Huo X et al. Identification of Novel Immunologic Checkpoint Gene Prognostic Markers for Ovarian Cancer. J Oncol 2022:8570882 (2022). PubMed: 36157232