Key features and details
- Rabbit polyclonal to TIL/TLR1
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-TIL/TLR1 antibody
See all TIL/TLR1 primary antibodies
DescriptionRabbit polyclonal to TIL/TLR1
Tested applicationsSuitable for: WB, IHC-P, Flow Cytmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat
- Human small intestine tissue; Human PBMCs; Ramos and Raw cell lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab189337 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 90 kDa.|
|IHC-P||Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|Flow Cyt||Use 0.5µg for 106 cells.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionParticipates in the innate immune response to microbial agents. Cooperates with TLR2 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.
Tissue specificityUbiquitous. Highly expressed in spleen, ovary, peripheral blood leukocytes, thymus and small intestine.
Sequence similaritiesBelongs to the Toll-like receptor family.
Contains 12 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 TIR domain.
Cellular localizationCell membrane. Cytoplasmic vesicle > phagosome membrane.
- Information by UniProt
- CD281 antibody
- CD281 antigen antibody
- KIAA0012 antibody
All lanes : Anti-TIL/TLR1 antibody (ab189337) at 2 µg/ml
Lane 1 : Ramos cell lysate
Lane 2 : Raw cell lysate
Lane 3 : TIL/TLR1 transfected HEK293 cell lysate
All lanes : Goat anti-rabbit HRP
Predicted band size: 90 kDa
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human small intestine tissue labeling TIL/TLR1 with ab189337 at 10 µg/ml.
Flow cytometric analysis of Human PBMCs (monocyte-gated) labeling TIL/TLR1 with ab189337 at 0.5 µg/106 cells. Shaded histogram represents cells without antibody, green represents isotype control, red represents ab189337. Goat anti-rabbit PE secondary antibody was used.
ab189337 has been referenced in 2 publications.
- Salyer ACD & David SA Transcriptomal signatures of vaccine adjuvants and accessory immunostimulation of sentinel cells by toll-like receptor 2/6 agonists. Hum Vaccin Immunother 14:1686-1696 (2018). PubMed: 29852079
- Huhta H et al. The Expression of Toll-like Receptors in Normal Human and Murine Gastrointestinal Organs and the Effect of Microbiome and Cancer. J Histochem Cytochem 64:470-82 (2016). PubMed: 27370795