Anti-TIL/TLR1 antibody (ab189337)
Key features and details
- Rabbit polyclonal to TIL/TLR1
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-TIL/TLR1 antibody
See all TIL/TLR1 primary antibodies -
Description
Rabbit polyclonal to TIL/TLR1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat -
Immunogen
Synthetic peptide within Human TIL/TLR1 aa 400-500 conjugated to keyhole limpet haemocyanin. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q15399 -
Positive control
- Human small intestine tissue; Human PBMCs; Ramos and Raw cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab189337 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (2) |
Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 90 kDa.
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IHC-P | (1) |
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Flow Cyt |
Use 0.5µg for 106 cells.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
Notes |
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WB
Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 90 kDa. |
IHC-P
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Flow Cyt
Use 0.5µg for 106 cells. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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Function
Participates in the innate immune response to microbial agents. Cooperates with TLR2 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. -
Tissue specificity
Ubiquitous. Highly expressed in spleen, ovary, peripheral blood leukocytes, thymus and small intestine. -
Sequence similarities
Belongs to the Toll-like receptor family.
Contains 12 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 TIR domain. -
Cellular localization
Cell membrane. Cytoplasmic vesicle > phagosome membrane. - Information by UniProt
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Database links
- Entrez Gene: 7096 Human
- Entrez Gene: 21897 Mouse
- Entrez Gene: 305354 Rat
- Omim: 601194 Human
- SwissProt: Q15399 Human
- SwissProt: Q9EPQ1 Mouse
- Unigene: 621817 Human
- Unigene: 654532 Human
see all -
Alternative names
- CD281 antibody
- CD281 antigen antibody
- KIAA0012 antibody
see all
Images
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All lanes : Anti-TIL/TLR1 antibody (ab189337) at 2 µg/ml
Lane 1 : Ramos cell lysate
Lane 2 : Raw cell lysate
Lane 3 : TIL/TLR1 transfected HEK293 cell lysate
Secondary
All lanes : Goat anti-rabbit HRP
Predicted band size: 90 kDa -
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human small intestine tissue labeling TIL/TLR1 with ab189337 at 10 µg/ml.
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Flow cytometric analysis of Human PBMCs (monocyte-gated) labeling TIL/TLR1 with ab189337 at 0.5 µg/106 cells. Shaded histogram represents cells without antibody, green represents isotype control, red represents ab189337. Goat anti-rabbit PE secondary antibody was used.
Protocols
Datasheets and documents
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Datasheet download
References (2)
ab189337 has been referenced in 2 publications.
- Salyer ACD & David SA Transcriptomal signatures of vaccine adjuvants and accessory immunostimulation of sentinel cells by toll-like receptor 2/6 agonists. Hum Vaccin Immunother 14:1686-1696 (2018). PubMed: 29852079
- Huhta H et al. The Expression of Toll-like Receptors in Normal Human and Murine Gastrointestinal Organs and the Effect of Microbiome and Cancer. J Histochem Cytochem 64:470-82 (2016). PubMed: 27370795