Key features and details
- Rabbit polyclonal to TIL/TLR1
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-TIL/TLR1 antibody
See all TIL/TLR1 primary antibodies
DescriptionRabbit polyclonal to TIL/TLR1
ab37068 recognises Toll-like receptor 1 (TIL/TLR1).
Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide corresponding to Human TIL/TLR1 (C terminal).
- Mouse spleen lysate IF/ICC: RAW246.7 cell line
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.02% Sodium azide
Concentration information loading...
Purification notesPurified by ion exchange chromatography
Our Abpromise guarantee covers the use of ab37068 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 90 kDa.|
|ICC/IF||Use at an assay dependent concentration.|
|IHC-P||Use a concentration of 10 µg/ml.|
FunctionParticipates in the innate immune response to microbial agents. Cooperates with TLR2 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.
Tissue specificityUbiquitous. Highly expressed in spleen, ovary, peripheral blood leukocytes, thymus and small intestine.
Sequence similaritiesBelongs to the Toll-like receptor family.
Contains 12 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 TIR domain.
Cellular localizationCell membrane. Cytoplasmic vesicle > phagosome membrane.
- Information by UniProt
- CD281 antibody
- CD281 antigen antibody
- KIAA0012 antibody
Lane 1 : Anti-TIL/TLR1 antibody (ab37068) at 1 µg/ml
Lane 2 : Anti-TIL/TLR1 antibody (ab37068) at 2 µg/ml
Lane 3 : Anti-TIL/TLR1 antibody (ab37068) at 4 µg/ml
All lanes : Mouse spleen lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 90 kDa
Additional bands at: 75 kDa. We are unsure as to the identity of these extra bands.
ab37068 at 10µg/ml staining TIL/TLR1 in mouse spleen tissue by IHC
ICC/IF image of ab37068 stained RAW246.7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab37068, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunofluorescence of TIL/TLR1 in Mouse Spleen cells using ab37068 at 20 ug/ml.
ab37068 has been referenced in 1 publication.
- Hinojosa E et al. Age-associated inflammation and toll-like receptor dysfunction prime the lungs for pneumococcal pneumonia. J Infect Dis 200:546-54 (2009). WB ; Mouse . PubMed: 19586419