Anti-TIM 1 antibody (ab47635)
Key features and details
- Rabbit polyclonal to TIM 1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
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Overview
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Product name
Anti-TIM 1 antibody
See all TIM 1 primary antibodies -
Description
Rabbit polyclonal to TIM 1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
A 16 amino acid peptide from near the amino terminus of human TIM 1.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab47635 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
Use a concentration of 1 - 8 µg/ml. Predicted molecular weight: 39 kDa.
Overnight incubation at 4°C is recommended. |
Notes |
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WB
Use a concentration of 1 - 8 µg/ml. Predicted molecular weight: 39 kDa. Overnight incubation at 4°C is recommended. |
Target
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Function
May play a role in T-helper cell development and the regulation of asthma and allergic diseases. Receptor for TIMD4 (By similarity). In case of human hepatitis A virus (HHAV) infection, functions as a cell-surface receptor for the virus. May play a role in kidney injury and repair. -
Tissue specificity
Widely expressed, with highest levels in kidney and testis. Expressed by activated CD4+ T-cells during the development of helper T-cells responses. -
Sequence similarities
Belongs to the immunoglobulin superfamily. TIM family.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 26762 Human
- Omim: 606518 Human
- SwissProt: Q96D42 Human
- Unigene: 129711 Human
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Alternative names
- CD365 antibody
- HAVCR 1 antibody
- HAVCR antibody
see all
Images
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All lanes : Anti-TIM 1 antibody (ab47635) at 8 µg/ml
Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
Lane 2 : A431 (human epidermoid carcinoma cell line) cell lysate
Lane 3 : A549 (human lung carcinoma cell line) cell lysate
Lane 4 : CaCo-2 (human colorectal adenocarcinoma cell line) cell lysate
Lane 5 : Daudi (human Burkitt's lymphoma cell line) cell lysate
Lane 6 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 7 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
Lane 8 : K562 (human chronic myelogenous leukemia cell line from bone marrow ) cell lysate
Lane 9 : MCF7 (human breast adenocarcinoma cell line) cell lysate
Lane 10 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
Lane 11 : SK-N-SH (human neuroblastoma cell line) cell lysate
Lane 12 : THP-1 (human monocytic leukemia cell line) cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-rabbit IgG (HRP) at 1/10000 dilution
Predicted band size: 39 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?Diluting buffer and concentration: 5% NFDM/TBST.
Primary incubated overnight at 4°C.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (30)
ab47635 has been referenced in 30 publications.
- Mizokami T et al. Macrophage Depletion Attenuates Acute Renal Damage after Exhaustive Exercise in Mice. Int J Sports Med 43:964-970 (2022). PubMed: 35426091
- Shankar S et al. Ex vivo-expanded human CD19+TIM-1+ regulatory B cells suppress immune responses in vivo and are dependent upon the TIM-1/STAT3 axis. Nat Commun 13:3121 (2022). PubMed: 35660734
- Lin TC et al. Juniperus communis extract ameliorates lipopolysaccharide-induced acute kidney injury through the adenosine monophosphate-activated protein kinase pathway. Food Sci Nutr 10:3405-3414 (2022). PubMed: 36249972
- Deng J et al. Cerium oxide-based nanozyme suppresses kidney calcium oxalate crystal depositions via reversing hyperoxaluria-induced oxidative stress damage. J Nanobiotechnology 20:516 (2022). PubMed: 36482378
- Guan Y et al. Protease-Activated Receptor 1 Contributes to Microcirculation Failure and Tubular Damage in Renal Ischemia-Reperfusion Injury in Mice. Biomed Res Int 2021:6665714 (2021). PubMed: 33681367