Overview

  • Product name
  • Description
    Rabbit polyclonal to TIM 1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    A 16 amino acid peptide from near the amino terminus of human TIM 1.

  • Positive control
    • Human Uterus Tissue Lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab47635 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 39 kDa.
IHC-P Use a concentration of 10 µg/ml.

Target

  • Function
    May play a role in T-helper cell development and the regulation of asthma and allergic diseases. Receptor for TIMD4 (By similarity). In case of human hepatitis A virus (HHAV) infection, functions as a cell-surface receptor for the virus. May play a role in kidney injury and repair.
  • Tissue specificity
    Widely expressed, with highest levels in kidney and testis. Expressed by activated CD4+ T-cells during the development of helper T-cells responses.
  • Sequence similarities
    Belongs to the immunoglobulin superfamily. TIM family.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD365 antibody
    • HAVCR 1 antibody
    • HAVCR antibody
    • HAVcr-1 antibody
    • Havcr1 antibody
    • Hepatitis A virus cellular receptor 1 antibody
    • Kidney injury molecule 1 antibody
    • KIM 1 antibody
    • KIM-1 antibody
    • T cell immunoglobin domain and mucin domain protein 1 antibody
    • T cell immunoglobulin mucin family member 1 antibody
    • T cell immunoglobulin mucin receptor 1 antibody
    • T-cell immunoglobulin and mucin domain-containing protein 1 antibody
    • T-cell membrane protein 1 antibody
    • TIM antibody
    • TIM-1 antibody
    • TIM1 antibody
    • TIMD 1 antibody
    • TIMD-1 antibody
    • TIMD1 antibody
    • TIMD1_HUMAN antibody
    see all

Images

  • Lane 1 : Anti-TIM 1 antibody (ab47635) at 1 µg/ml
    Lane 2 : Anti-TIM 1 antibody (ab47635) at 2 µg/ml

    All lanes : Human Uterus Tissue Lysate

    Lysates/proteins at 15 µg per lane.

    Predicted band size: 39 kDa
    Observed band size: 50 kDa
    why is the actual band size different from the predicted?

  • Ab47635 at 5μg/ml staining TIM1 in normal human kidney tissue section by Immunohistochemistry ( IHC-P-Formalin/PFA fixed paraffin-embedded sections).

  • Ab47635 at 5μg/ml staining TIM1 in human kidney carcinoma tissue section by Immunohistochemistry (IHC-P-Formalin/PFA fixed paraffin-embedded sections).

  • ICC/IF image of ab47635 stained HeLa cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab47635, 5µg/ml) overnight at +4°C. The secondary antibody (green) was for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:
  • Ramani K  et al. Unexpected kidney-restricted role for IL-17 receptor signaling in defense against systemic Candida albicans infection. JCI Insight 3:N/A (2018). Read more (PubMed: 29720566) »
  • Joshi S  et al. Transcriptional study of hyperoxaluria and calcium oxalate nephrolithiasis in male rats: Inflammatory changes are mainly associated with crystal deposition. PLoS One 12:e0185009 (2017). Read more (PubMed: 29091707) »
See all 2 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer


Product ab47635 was raised using N-terminal peptide and immunogen corresponds to the ecto domain of protein Tim 1 (http://www.uniprot.org/uniprot/Q96D42). This antibody has been tested with mouse and human samples in WB, ICC/IF and IHC-P.

Rat species is yet to be tested for this antibody so if you are interested, we can offer you Abtrial for rat species. The details can be found at the following link;

https://www.abcam.com/index.html?pageconfig=resource&rid=11998&viapagetrap=collaborationdiscount

Read More

Answer

Thank you for contacting abcam.

The image on our datasheet for this product ddoes show high non-specific background as well which seemed to be tamed but not eliminated by using a much lower dilution of the product. In that particular case 1ug/ml versus 2ug/ml with 15ug of total protein per well. Althought the exact concentrations will be different with each protocol and tissue type the principle should hold. I would recommend that you titer the ideal antibody concentration at a much lower level.

Another recommendation is to use a BSA block before and with your primary antibody but to use a casein based commercial block for incubating your secondary antibody. We have seen that this may dramatically reduce background in western blot. We do carry this type of product:

Background Reducing Buffer (https://www.abcam.com/Background-Reducing-Buffer-ab64234.html)

Please let me know if you have any questions or there are other ways that Abcam may help you meet your research goals.

We do offer a

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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