Product nameAnti-TIM 1 antibody
See all TIM 1 primary antibodies
DescriptionRabbit polyclonal to TIM 1
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
A 16 amino acid peptide from near the amino terminus of human TIM 1.
- Human Uterus Tissue Lysate.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.02% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab47635 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|WB||Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 39 kDa.|
|IHC-P||Use a concentration of 10 µg/ml.|
FunctionMay play a role in T-helper cell development and the regulation of asthma and allergic diseases. Receptor for TIMD4 (By similarity). In case of human hepatitis A virus (HHAV) infection, functions as a cell-surface receptor for the virus. May play a role in kidney injury and repair.
Tissue specificityWidely expressed, with highest levels in kidney and testis. Expressed by activated CD4+ T-cells during the development of helper T-cells responses.
Sequence similaritiesBelongs to the immunoglobulin superfamily. TIM family.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
- Information by UniProt
- CD365 antibody
- HAVCR 1 antibody
- HAVCR antibody
Lane 1 : Anti-TIM 1 antibody (ab47635) at 1 µg/ml
Lane 2 : Anti-TIM 1 antibody (ab47635) at 2 µg/ml
All lanes : Human Uterus Tissue Lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 39 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Ab47635 at 5μg/ml staining TIM1 in normal human kidney tissue section by Immunohistochemistry ( IHC-P-Formalin/PFA fixed paraffin-embedded sections).
Ab47635 at 5μg/ml staining TIM1 in human kidney carcinoma tissue section by Immunohistochemistry (IHC-P-Formalin/PFA fixed paraffin-embedded sections).
ICC/IF image of ab47635 stained HeLa cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab47635, 5µg/ml) overnight at +4°C. The secondary antibody (green) was for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
This product has been referenced in:
- Ramani K et al. Unexpected kidney-restricted role for IL-17 receptor signaling in defense against systemic Candida albicans infection. JCI Insight 3:N/A (2018). Read more (PubMed: 29720566) »
- Joshi S et al. Transcriptional study of hyperoxaluria and calcium oxalate nephrolithiasis in male rats: Inflammatory changes are mainly associated with crystal deposition. PLoS One 12:e0185009 (2017). Read more (PubMed: 29091707) »