Product nameAnti-TIM 1 antibody
See all TIM 1 primary antibodies
DescriptionMouse monoclonal to TIM 1
Tested applicationsSuitable for: WB, ELISA, Flow Cytmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee
- TIM 1 transfected 293T cell lysate.
This product was changed from ascites to tissue culture supernatant on 24th June 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: None
Constituents: PBS, pH 7.2
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab66062 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Detects a band of approximately 36 kDa (predicted molecular weight: 39 kDa).|
|ELISA||Use at an assay dependent concentration. The detection limit for recombinant tagged TIM 1 is approximately 10ng/ml if used as a capture antibody.|
|Flow Cyt||Use at an assay dependent concentration.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
FunctionMay play a role in T-helper cell development and the regulation of asthma and allergic diseases. Receptor for TIMD4 (By similarity). In case of human hepatitis A virus (HHAV) infection, functions as a cell-surface receptor for the virus. May play a role in kidney injury and repair.
Tissue specificityWidely expressed, with highest levels in kidney and testis. Expressed by activated CD4+ T-cells during the development of helper T-cells responses.
Sequence similaritiesBelongs to the immunoglobulin superfamily. TIM family.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
- Information by UniProt
- CD365 antibody
- HAVCR 1 antibody
- HAVCR antibody
All lanes : Anti-TIM 1 antibody (ab66062) at 1 µg/ml
Lane 1 : TIM 1 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 50 µg per lane.
All lanes : Goat Anti-Mouse IgG at 1/2500 dilution
Predicted band size: 39 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted?
This image was generated using the ascites version of the product.
This product has been referenced in:
- Xiao X et al. Aldosterone induces NRK-52E cell apoptosis in acute kidney injury via rno-miR-203 hypermethylation and Kim-1 upregulation. Exp Ther Med 12:915-924 (2016). WB ; Mouse . Read more (PubMed: 27446296) »
- Liu J et al. IL-10-producing B cells are induced early in HIV-1 infection and suppress HIV-1-specific T cell responses. PLoS One 9:e89236 (2014). Flow Cyt . Read more (PubMed: 24586620) »