Key features and details
- Mouse monoclonal to TIM 1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG2a
Product nameAnti-TIM 1 antibody
See all TIM 1 primary antibodies
DescriptionMouse monoclonal to TIM 1
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee
- TIM 1 transfected 293T cell lysate.
This product was changed from ascites to tissue culture supernatant on 24th June 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.4
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab66062 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Detects a band of approximately 36 kDa (predicted molecular weight: 39 kDa).|
FunctionMay play a role in T-helper cell development and the regulation of asthma and allergic diseases. Receptor for TIMD4 (By similarity). In case of human hepatitis A virus (HHAV) infection, functions as a cell-surface receptor for the virus. May play a role in kidney injury and repair.
Tissue specificityWidely expressed, with highest levels in kidney and testis. Expressed by activated CD4+ T-cells during the development of helper T-cells responses.
Sequence similaritiesBelongs to the immunoglobulin superfamily. TIM family.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
- Information by UniProt
- CD365 antibody
- HAVCR 1 antibody
- HAVCR antibody
All lanes : Anti-TIM 1 antibody (ab66062) at 1 µg/ml
Lane 1 : TIM 1 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 50 µg per lane.
All lanes : Goat Anti-Mouse IgG at 1/2500 dilution
Predicted band size: 39 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted?
This image was generated using the ascites version of the product.
ab66062 has been referenced in 3 publications.
- Hu Q et al. 17ß-Estradiol treatment drives Sp1 to upregulate MALAT-1 expression and epigenetically affects physiological processes in U2OS cells. Mol Med Rep 15:1335-1342 (2017). PubMed: 28098894
- Xiao X et al. Aldosterone induces NRK-52E cell apoptosis in acute kidney injury via rno-miR-203 hypermethylation and Kim-1 upregulation. Exp Ther Med 12:915-924 (2016). WB ; Mouse . PubMed: 27446296
- Liu J et al. IL-10-producing B cells are induced early in HIV-1 infection and suppress HIV-1-specific T cell responses. PLoS One 9:e89236 (2014). Flow Cyt . PubMed: 24586620