Anti-TIM 1 antibody (ab66062)

Reviews (1)Q&A (1)References (3)
Western blot - Anti-TIM 1 antibody (ab66062)

    Key features and details

    • Mouse monoclonal to TIM 1
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG2a

    Overview

    • Product name

      Anti-TIM 1 antibody
      See all TIM 1 primary antibodies

    • Description

      Mouse monoclonal to TIM 1

    • Host species

      Mouse

    • Tested applications

      Suitable for: WBmore details

    • Species reactivity

      Reacts with: Human
      Predicted to work with: Chimpanzee

    • Immunogen

      Recombinant fragment with tag: KVGGEAGPSV TLPCHYSGAV TSMCWNRGSC SLFTCQNGIV WTNGTHVTYR KDTRYKLLGD LSRRDVSLTI ENTAVSDSGV YCCRVEHRGW FNDMKITVSL, corresponding to amino acids 23-123 of Human TIM 1

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • Positive control

      • TIM 1 transfected 293T cell lysate.

    • General notes

      This product was changed from ascites to tissue culture supernatant on 24th June 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    Applications

    Our Abpromise guarantee covers the use of ab66062 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB Use at an assay dependent concentration. Detects a band of approximately 36 kDa (predicted molecular weight: 39 kDa).

    Target

    • Function

      May play a role in T-helper cell development and the regulation of asthma and allergic diseases. Receptor for TIMD4 (By similarity). In case of human hepatitis A virus (HHAV) infection, functions as a cell-surface receptor for the virus. May play a role in kidney injury and repair.

    • Tissue specificity

      Widely expressed, with highest levels in kidney and testis. Expressed by activated CD4+ T-cells during the development of helper T-cells responses.

    • Sequence similarities

      Belongs to the immunoglobulin superfamily. TIM family.
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.

    • Cellular localization

      Membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • CD365 antibody
        • HAVCR 1 antibody
        • HAVCR antibody
        • HAVcr-1 antibody
        • Havcr1 antibody
        • Hepatitis A virus cellular receptor 1 antibody
        • Kidney injury molecule 1 antibody
        • KIM 1 antibody
        • KIM-1 antibody
        • T cell immunoglobin domain and mucin domain protein 1 antibody
        • T cell immunoglobulin mucin family member 1 antibody
        • T cell immunoglobulin mucin receptor 1 antibody
        • T-cell immunoglobulin and mucin domain-containing protein 1 antibody
        • T-cell membrane protein 1 antibody
        • TIM antibody
        • TIM-1 antibody
        • TIM1 antibody
        • TIMD 1 antibody
        • TIMD-1 antibody
        • TIMD1 antibody
        • TIMD1_HUMAN antibody
        see all

      Images

      • Western blot - Anti-TIM 1 antibody (ab66062)
        Western blot - Anti-TIM 1 antibody (ab66062)
        All lanes : Anti-TIM 1 antibody (ab66062) at 1 µg/ml

        Lane 1 : TIM 1 transfected 293T cell lysate
        Lane 2 : Non-transfected 293T cell lysate

        Lysates/proteins at 50 µg per lane.

        Secondary
        All lanes : Goat Anti-Mouse IgG at 1/2500 dilution

        Predicted band size: 39 kDa
        Observed band size: 36 kDa
        why is the actual band size different from the predicted?



        This image was generated using the ascites version of the product.

      References (3)

      Publishing research using ab66062? Please let us know so that we can cite the reference in this datasheet.

      ab66062 has been referenced in 3 publications.

      • Hu Q  et al. 17ß-Estradiol treatment drives Sp1 to upregulate MALAT-1 expression and epigenetically affects physiological processes in U2OS cells. Mol Med Rep 15:1335-1342 (2017). PubMed: 28098894
      • Xiao X  et al. Aldosterone induces NRK-52E cell apoptosis in acute kidney injury via rno-miR-203 hypermethylation and Kim-1 upregulation. Exp Ther Med 12:915-924 (2016). WB ; Mouse . PubMed: 27446296
      • Liu J  et al. IL-10-producing B cells are induced early in HIV-1 infection and suppress HIV-1-specific T cell responses. PLoS One 9:e89236 (2014). Flow Cyt . PubMed: 24586620

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a question

      Question

       My name is Johnny Huang and I’m from the University of Queensland, Australia. I purchased your antibodies against TIM-1 (ab66062 and 47634) recently. I tried the antibodies for western blotting but I couldn’t get single band. In fact, TIM-1 is a glycoprotein, and if you run a SDS-PAGE with purified TIM-1 protein, you will get a smear view on the PAGE. I noticed you have an example picture on your website, w+hich shows one single band  on western blotting. https://www.abcam.com/TIM-1-antibody-ab66062.html Would you please let me know the conditions you use for the preparation of tissue lysis and western blotting in detail? Did you perform a de-glycosylation step before the western blotting? Thank you very much!   Regards,

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      Answer

      Thank you for contacting us. We find that specific protocol is not necessary for this product. So I have attached the protocol that we generally recommend to our customers. We have used reduced denaturing conditions in WB without deglycosylation step. I can further help you with troubleshooting if you can provide more details about the protocol. Please find attached the questionnaire that we have created to get more information about the protocol customer use. Please fill in the form and send it to me via email asap. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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