Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17561-22] to TIM 1 - BSA and Azide free
- Suitable for: Flow Cyt
- Reacts with: Mouse
Product nameAnti-TIM 1 antibody [EPR17561-22] - BSA and Azide free
See all TIM 1 primary antibodies
DescriptionRabbit monoclonal [EPR17561-22] to TIM 1 - BSA and Azide free
Tested applicationsSuitable for: Flow Cytmore details
Unsuitable for: ICC/IF,IHC-P or WB
Species reactivityReacts with: Mouse
Recombinant fragment within Mouse TIM 1 aa 1-250. The exact sequence is proprietary.
Database link: Q5QNS5
- Flow cyt: A20 cells.
Ab252385 is the carrier-free version of ab252220. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab252385 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Storage bufferpH: 7.2
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab252385 in the following tested applications.
|Flow Cyt||Use at an assay dependent concentration.|
FunctionMay play a role in T-helper cell development and the regulation of asthma and allergic diseases. Receptor for TIMD4 (By similarity). In case of human hepatitis A virus (HHAV) infection, functions as a cell-surface receptor for the virus. May play a role in kidney injury and repair.
Tissue specificityWidely expressed, with highest levels in kidney and testis. Expressed by activated CD4+ T-cells during the development of helper T-cells responses.
Sequence similaritiesBelongs to the immunoglobulin superfamily. TIM family.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
- Information by UniProt
- CD365 antibody
- HAVCR 1 antibody
- HAVCR antibody
Flow cytometric analysis of Mouse splenocytes (Left panel) / A20 (mouse reticulum sarcoma cell line, Right panel) labeling TIM 1 with ab252220 at 1/50 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.
Negative control: Mouse splenocytes (PMID: 26332704; PMID: 17277130).
Gated on viable cells.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252220).
ab252385 has not yet been referenced specifically in any publications.