Product nameAnti-TIM 1 antibody [EPR22650-136]
See all TIM 1 primary antibodies
DescriptionRabbit monoclonal [EPR22650-136] to TIM 1
Tested applicationsSuitable for: WB, ICC/IF, Flow Cytmore details
Unsuitable for: IHC-P or IP
Species reactivityReacts with: Human
Recombinant fragment within Human TIM 1 aa 1-150. The exact sequence is proprietary.
Database link: Q96D42
- WB: Huh7 whole cell lysate. ICC/IF: Huh7 cells. Flow Cyt: Huh7 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab228973 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 39 kDa.|
FunctionMay play a role in T-helper cell development and the regulation of asthma and allergic diseases. Receptor for TIMD4 (By similarity). In case of human hepatitis A virus (HHAV) infection, functions as a cell-surface receptor for the virus. May play a role in kidney injury and repair.
Tissue specificityWidely expressed, with highest levels in kidney and testis. Expressed by activated CD4+ T-cells during the development of helper T-cells responses.
Sequence similaritiesBelongs to the immunoglobulin superfamily. TIM family.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
- Information by UniProt
- CD365 antibody
- HAVCR 1 antibody
- HAVCR antibody
All lanes : Anti-TIM 1 antibody [EPR22650-136] (ab228973) at 1/1000 dilution
Lane 1 : Huh7 (human hepatocellular carcinoma epithelial cell), whole cell lysate
Lane 2 : 293T (human embryonic kidney epithelial cell), whole cell lysate
Lane 3 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 39 kDa
Observed band size: 140,70 kDa why is the actual band size different from the predicted?
Exposure time: 15 seconds
Negative control: 293T and HeLa (PMID: 23084921).
TIM1 is often observed a band with a range of 90-140kDa due to glycosylation.
Blocking/Dilution buffer: 5% NFDM/TBST.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Huh7 (human hepatocellular carcinoma epithelial cell) cells labeling TIM 1 with ab228973 at 1/100 (5.3µg/ml) dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 (2µg/ml) dilution (Green). Confocal image showing cytoplasmic punctate and membranous staining in Huh7 cell line (PMID:23233528) is observed. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).
Secondary antibody only control/ Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution.
Negative control: HEK-293T（PMID: 23084921).
Flow cytometric analysis of 293T (human embryonic kidney epithelial cell, Left) / Huh7 (human hepatocellular carcinoma epithelial cell, Right) cells labeling TIM 1 with ab228973 at 1/500 compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells. Negative control: HEK-293T (PMID: 23084921).
ab228973 has not yet been referenced specifically in any publications.