Recombinant Anti-TIM 1 antibody [EPR22650-136] (ab228973)


  • Product name

    Anti-TIM 1 antibody [EPR22650-136]
    See all TIM 1 primary antibodies
  • Description

    Rabbit monoclonal [EPR22650-136] to TIM 1
  • Host species

  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cytmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human TIM 1 aa 1-150. The exact sequence is proprietary.
    Database link: Q96D42

  • Positive control

    • WB: Huh7 whole cell lysate. ICC/IF: Huh7 cells. Flow Cyt: Huh7 cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab228973 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 39 kDa.
ICC/IF 1/100.
Flow Cyt 1/500.
  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function

      May play a role in T-helper cell development and the regulation of asthma and allergic diseases. Receptor for TIMD4 (By similarity). In case of human hepatitis A virus (HHAV) infection, functions as a cell-surface receptor for the virus. May play a role in kidney injury and repair.
    • Tissue specificity

      Widely expressed, with highest levels in kidney and testis. Expressed by activated CD4+ T-cells during the development of helper T-cells responses.
    • Sequence similarities

      Belongs to the immunoglobulin superfamily. TIM family.
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    • Cellular localization

    • Information by UniProt
    • Database links

    • Alternative names

      • CD365 antibody
      • HAVCR 1 antibody
      • HAVCR antibody
      • HAVcr-1 antibody
      • Havcr1 antibody
      • Hepatitis A virus cellular receptor 1 antibody
      • Kidney injury molecule 1 antibody
      • KIM 1 antibody
      • KIM-1 antibody
      • T cell immunoglobin domain and mucin domain protein 1 antibody
      • T cell immunoglobulin mucin family member 1 antibody
      • T cell immunoglobulin mucin receptor 1 antibody
      • T-cell immunoglobulin and mucin domain-containing protein 1 antibody
      • T-cell membrane protein 1 antibody
      • TIM antibody
      • TIM-1 antibody
      • TIM1 antibody
      • TIMD 1 antibody
      • TIMD-1 antibody
      • TIMD1 antibody
      • TIMD1_HUMAN antibody
      see all


    • All lanes : Anti-TIM 1 antibody [EPR22650-136] (ab228973) at 1/1000 dilution

      Lane 1 : Huh7 (human hepatocellular carcinoma epithelial cell), whole cell lysate
      Lane 2 : 293T (human embryonic kidney epithelial cell), whole cell lysate
      Lane 3 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate

      Lysates/proteins at 20 µg per lane.

      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 39 kDa
      Observed band size: 140,70 kDa
      why is the actual band size different from the predicted?

      Exposure time: 15 seconds

      Negative control: 293T and HeLa (PMID: 23084921).

      TIM1 is often observed a band with a range of 90-140kDa due to glycosylation.

      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Huh7 (human hepatocellular carcinoma epithelial cell) cells labeling TIM 1 with ab228973 at 1/100 (5.3µg/ml) dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 (2µg/ml) dilution (Green). Confocal image showing cytoplasmic punctate and membranous staining in Huh7 cell line (PMID:23233528) is observed. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

      Secondary antibody only control/ Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution.

      Negative control: HEK-293T(PMID: 23084921).

    • Flow cytometric analysis of 293T (human embryonic kidney epithelial cell, Left) / Huh7 (human hepatocellular carcinoma epithelial cell, Right) cells labeling TIM 1 with ab228973 at 1/500 compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells. Negative control: HEK-293T (PMID: 23084921).


    ab228973 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab228973.
    Please use the links above to contact us or submit feedback about this product.

    For licensing inquiries, please contact

    Sign up