Recombinant
RabMAb

Recombinant Anti-TIM 1 antibody [EPR22650-136] (ab228973)

Overview

  • Product name

    Anti-TIM 1 antibody [EPR22650-136]
    See all TIM 1 primary antibodies
  • Description

    Rabbit monoclonal [EPR22650-136] to TIM 1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cytmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human TIM 1 aa 1-150. The exact sequence is proprietary.
    Database link: Q96D42

  • Positive control

    • WB: Huh7 whole cell lysate. ICC/IF: Huh7 cells. Flow Cyt: Huh7 cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab228973 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 39 kDa.
ICC/IF 1/100.
Flow Cyt 1/500.
  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function

      May play a role in T-helper cell development and the regulation of asthma and allergic diseases. Receptor for TIMD4 (By similarity). In case of human hepatitis A virus (HHAV) infection, functions as a cell-surface receptor for the virus. May play a role in kidney injury and repair.
    • Tissue specificity

      Widely expressed, with highest levels in kidney and testis. Expressed by activated CD4+ T-cells during the development of helper T-cells responses.
    • Sequence similarities

      Belongs to the immunoglobulin superfamily. TIM family.
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    • Cellular localization

      Membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • CD365 antibody
      • HAVCR 1 antibody
      • HAVCR antibody
      • HAVcr-1 antibody
      • Havcr1 antibody
      • Hepatitis A virus cellular receptor 1 antibody
      • Kidney injury molecule 1 antibody
      • KIM 1 antibody
      • KIM-1 antibody
      • T cell immunoglobin domain and mucin domain protein 1 antibody
      • T cell immunoglobulin mucin family member 1 antibody
      • T cell immunoglobulin mucin receptor 1 antibody
      • T-cell immunoglobulin and mucin domain-containing protein 1 antibody
      • T-cell membrane protein 1 antibody
      • TIM antibody
      • TIM-1 antibody
      • TIM1 antibody
      • TIMD 1 antibody
      • TIMD-1 antibody
      • TIMD1 antibody
      • TIMD1_HUMAN antibody
      see all

    Images

    • All lanes : Anti-TIM 1 antibody [EPR22650-136] (ab228973) at 1/1000 dilution

      Lane 1 : Huh7 (human hepatocellular carcinoma epithelial cell), whole cell lysate
      Lane 2 : 293T (human embryonic kidney epithelial cell), whole cell lysate
      Lane 3 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 39 kDa
      Observed band size: 140,70 kDa
      why is the actual band size different from the predicted?


      Exposure time: 15 seconds


      Negative control: 293T and HeLa (PMID: 23084921).

      TIM1 is often observed a band with a range of 90-140kDa due to glycosylation.

      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Huh7 (human hepatocellular carcinoma epithelial cell) cells labeling TIM 1 with ab228973 at 1/100 (5.3µg/ml) dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 (2µg/ml) dilution (Green). Confocal image showing cytoplasmic punctate and membranous staining in Huh7 cell line (PMID:23233528) is observed. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

      Secondary antibody only control/ Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution.

      Negative control: HEK-293T(PMID: 23084921).

    • Flow cytometric analysis of 293T (human embryonic kidney epithelial cell, Left) / Huh7 (human hepatocellular carcinoma epithelial cell, Right) cells labeling TIM 1 with ab228973 at 1/500 compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells. Negative control: HEK-293T (PMID: 23084921).

    References

    ab228973 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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