Anti-TIM 1 antibody [mAbcam75295] (ab75295)

Overview

  • Product name
    Anti-TIM 1 antibody [mAbcam75295]
    See all TIM 1 primary antibodies
  • Description
    Mouse monoclonal [mAbcam75295] to TIM 1
  • Host species
    Mouse
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide derived from within residues 300 to the C-terminus of Human TIM 1.

    (Peptide available as ab97979.)

  • Positive control
    • This antibody gave a positive signal in the human testis and brain tissue lysates.
  • General notes

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

Applications

Our Abpromise guarantee covers the use of ab75295 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 10 mg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 39 kDa).

Target

  • Function
    May play a role in T-helper cell development and the regulation of asthma and allergic diseases. Receptor for TIMD4 (By similarity). In case of human hepatitis A virus (HHAV) infection, functions as a cell-surface receptor for the virus. May play a role in kidney injury and repair.
  • Tissue specificity
    Widely expressed, with highest levels in kidney and testis. Expressed by activated CD4+ T-cells during the development of helper T-cells responses.
  • Sequence similarities
    Belongs to the immunoglobulin superfamily. TIM family.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD365 antibody
    • HAVCR 1 antibody
    • HAVCR antibody
    • HAVcr-1 antibody
    • Havcr1 antibody
    • Hepatitis A virus cellular receptor 1 antibody
    • Kidney injury molecule 1 antibody
    • KIM 1 antibody
    • KIM-1 antibody
    • T cell immunoglobin domain and mucin domain protein 1 antibody
    • T cell immunoglobulin mucin family member 1 antibody
    • T cell immunoglobulin mucin receptor 1 antibody
    • T-cell immunoglobulin and mucin domain-containing protein 1 antibody
    • T-cell membrane protein 1 antibody
    • TIM antibody
    • TIM-1 antibody
    • TIM1 antibody
    • TIMD 1 antibody
    • TIMD-1 antibody
    • TIMD1 antibody
    • TIMD1_HUMAN antibody
    see all

Images

  • All lanes : Anti-TIM 1 antibody [mAbcam75295] (ab75295) at 10 µg/ml

    Lane 1 : Human testis tissue lysate - total protein (ab30257)
    Lane 2 : Human brain tissue lysate - total protein (ab29466)

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 39 kDa
    Observed band size: 50 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 35 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 90 seconds


    The band at approximately 50-kDa is comparable to the molecular weight seen with other antibodies to TIM 1. TIM 1 contains a number of potential glycosylation sites (SwissProt), which may explain its migration at a higher molecular weight than predicted.

References

ab75295 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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