Key features and details
- Mouse monoclonal [mAbcam75295] to TIM 1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-TIM 1 antibody [mAbcam75295]
See all TIM 1 primary antibodies
DescriptionMouse monoclonal [mAbcam75295] to TIM 1
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human TIM 1 aa 300 to the C-terminus (C terminal).
(Peptide available as
- This antibody gave a positive signal in the human testis and brain tissue lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituent: 1% BSA
Concentration information loading...
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab75295 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 10 mg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 39 kDa).|
FunctionMay play a role in T-helper cell development and the regulation of asthma and allergic diseases. Receptor for TIMD4 (By similarity). In case of human hepatitis A virus (HHAV) infection, functions as a cell-surface receptor for the virus. May play a role in kidney injury and repair.
Tissue specificityWidely expressed, with highest levels in kidney and testis. Expressed by activated CD4+ T-cells during the development of helper T-cells responses.
Sequence similaritiesBelongs to the immunoglobulin superfamily. TIM family.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
- Information by UniProt
- CD365 antibody
- HAVCR 1 antibody
- HAVCR antibody
All lanes : Anti-TIM 1 antibody [mAbcam75295] (ab75295) at 10 µg/ml
Lane 1 : Human testis tissue lysate - total protein (ab30257)
Lane 2 : Human brain tissue lysate - total protein (ab29466)
Lysates/proteins at 20 µg per lane.
All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 39 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Additional bands at: 35 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 90 seconds
The band at approximately 50-kDa is comparable to the molecular weight seen with other antibodies to TIM 1. TIM 1 contains a number of potential glycosylation sites (SwissProt), which may explain its migration at a higher molecular weight than predicted.
ab75295 has not yet been referenced specifically in any publications.