Product nameTIM 1 overexpression 293T lysate (whole cell)
ab94224 is a 293T cell transfected lysate in which Human TIM 1 has been transiently over-expressed using a pCMV-TIM 1 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more detailed how the transfected lysate was prepared view preparation notes
Tested applicationsSuitable for: WBmore details
Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
Storage bufferConstituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine)
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BackgroundFunction: May play a role in T-helper cell development and the regulation of asthma and allergic diseases. Receptor for TIMD4 (By similarity). In case of human hepatitis A virus (HHAV) infection, functions as a cell-surface receptor for the virus. May play a role in kidney injury and repair. Tissue specificity: Widely expressed, with highest levels in kidney and testis. Expressed by activated CD4+ T-cells during the development of helper T-cells responses. Similarity: Belongs to the immunoglobulin superfamily. TIM family. Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Our Abpromise guarantee covers the use of ab94224 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution.|
ab94224 at 15µg/lane on an SDS-PAGE gel.
All lanes : Anti-TIM 1 antibody (ab66062) at 1/500 dilution
Lane 1 :
TIM 1 overexpression 293T lysate (whole cell) (ab94224)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
ab94224 has not yet been referenced specifically in any publications.