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    tim-4-antibody-ab47636.pdf

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Immunology Adaptive Immunity T Cells Non-CD
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Anti-TIM 4 antibody (ab47636)

  • Datasheet
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Abpromise

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Western blot - Anti-TIM 4 antibody (ab47636)

    Key features and details

    • Rabbit polyclonal to TIM 4
    • Suitable for: WB
    • Reacts with: Mouse, Rat, Human
    • Isotype: IgG

    You may also be interested in

    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    Protein
    Recombinant mouse TIM 4 protein (Fc Chimera Active) (ab214993)

    View more associated products

    Overview

    • Product name

      Anti-TIM 4 antibody
      See all TIM 4 primary antibodies
    • Description

      Rabbit polyclonal to TIM 4
    • Host species

      Rabbit
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Mouse, Rat, Human
    • Immunogen

      A 15 amino acid peptide from near the carboxy terminus of human TIM 4.

    • Positive control

      • Jurkat cells lysate
    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C.
    • Storage buffer

      pH: 7.2
      Preservative: 0.02% Sodium azide
      Constituent: PBS
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Immunology
      • Adaptive Immunity
      • T Cells
      • Non-CD

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Positive Controls

      • Jurkat membrane extract lysate (ab30130)
    • Recombinant Protein

      • Recombinant mouse TIM 4 protein (Fc Chimera Active) (ab214993)

    Applications

    Our Abpromise guarantee covers the use of ab47636 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 42 kDa.

    Target

    • Function

      Phosphatidylserine receptor that enhances the engulfment of apoptotic cells. Involved in regulating T-cell proliferation and lymphotoxin signaling. Ligand for HAVCR1/TIMD1.
    • Sequence similarities

      Belongs to the immunoglobulin superfamily. TIM family.
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    • Domain

      Recognizes phosphatidyl serine via its immunoglobulin domain.
    • Cellular localization

      Membrane.
    • Target information above from: UniProt accession Q96H15 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 91937 Human
      • Entrez Gene: 276891 Mouse
      • Entrez Gene: 497891 Rat
      • Omim: 610096 Human
      • SwissProt: Q96H15 Human
      • SwissProt: Q6U7R4 Mouse
      • Unigene: 334907 Human
      • Unigene: 69002 Mouse
      • Alternative names

        • SMUCKLER antibody
        • Spleen, mucin-containing, knockout of lymphotoxin protein antibody
        • T cell immunoglobulin and mucin domain containing protein 4 antibody
        • T-cell immunoglobulin and mucin domain containing 4 antibody
        • T-cell immunoglobulin and mucin domain containing molecule antibody
        • T-cell immunoglobulin and mucin domain-containing protein 4 antibody
        • T-cell immunoglobulin and mucin domains-containing protein 4 antibody
        • T-cell membrane protein 4 antibody
        • TIM-4 antibody
        • Tim4 antibody
        • TIMD 4 antibody
        • TIMD-4 antibody
        • Timd4 antibody
        • TIMD4_HUMAN antibody
        see all

      Images

      • Western blot - Anti-TIM 4 antibody (ab47636)
        Western blot - Anti-TIM 4 antibody (ab47636)
        Lane 1 : Anti-TIM 4 antibody (ab47636) at 1 µg/ml
        Lane 2 : Anti-TIM 4 antibody (ab47636) at 2 µg/ml

        All lanes : Jurkat Lysate

        Lysates/proteins at 15 µg per lane.

        Predicted band size: 42 kDa
        Observed band size: 50 kDa
        why is the actual band size different from the predicted?

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (0)

      Publishing research using ab47636? Please let us know so that we can cite the reference in this datasheet.

      ab47636 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a question

      Question

      Is the immunogen extracellular or intracellular? I'm using this in FC.

      Read More

      Abcam community

      Verified customer

      Asked on Aug 30 2011

      Answer

      Thank you for your inquiry. I heard back from the supplying lab that ab47637 is against a peptide from the extracellular portion of TIM4. In case you need to know, the other one in our catalogue, ab47636, is against the cytoplasmic domain. I hope this information helps. Please contact us with any other questions.

      Read More

      Abcam Scientific Support

      Answered on Aug 30 2011

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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