Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22304-3] to TIM 4
- Suitable for: ICC/IF, Flow Cyt
- Reacts with: Human
Product nameAnti-TIM 4 antibody [EPR22304-3]
See all TIM 4 primary antibodies
DescriptionRabbit monoclonal [EPR22304-3] to TIM 4
Tested applicationsSuitable for: ICC/IF, Flow Cytmore details
Unsuitable for: IHC-P,IP or WB
Species reactivityReacts with: Human
Recombinant fragment within Human TIM 4 aa 1-150. The exact sequence is proprietary.
Database link: Q96H15
- ICC/IF: HEK-293T cells. Flow Cyt: HEK-293T cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab222093 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
FunctionPhosphatidylserine receptor that enhances the engulfment of apoptotic cells. Involved in regulating T-cell proliferation and lymphotoxin signaling. Ligand for HAVCR1/TIMD1.
Sequence similaritiesBelongs to the immunoglobulin superfamily. TIM family.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
DomainRecognizes phosphatidyl serine via its immunoglobulin domain.
- Information by UniProt
- SMUCKLER antibody
- Spleen, mucin-containing, knockout of lymphotoxin protein antibody
- T cell immunoglobulin and mucin domain containing protein 4 antibody
Flow cytometric analysis of 4% paraformaldehyde-fixed HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with a myc-tagged TIM 4 expression construct labeling TIM 4 with ab222093 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730, Black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody, Blue).
Cells were surface stained with ab222093. Then fixed with 2% PFA for 10min, followed by intracellular staining of anti-myc tag conjugated to Alexa Fluor®647.
Gated on myc -positive population.
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized TIM 4 transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells labeling TIM 4 (Green) with ab222093 at 1/100 dilution, followed by AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) antibody at 1/1000 dilution. Confocal image showing positive staining in HEK-293T cells transfected with a myc-tagged TIM 4 expression construct. myc-Tag (9B11) Mouse mAb (Alexa Fluor® 594 Conjugate) (ab223894) was used as the counterstain (Red) at 1/100 dilution. The nuclear counterstain was DAPI (Blue).
Negative control: Myc only transfected HEK-293T cells.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is AlexaFluor®488 Goat anti-Rabbit secondary (ab150077).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab222093 has not yet been referenced specifically in any publications.