Recombinant
RabMAb

Recombinant Anti-TIM 4 antibody [EPR22304-3] (ab222093)

Overview

  • Product name

    Anti-TIM 4 antibody [EPR22304-3]
    See all TIM 4 primary antibodies
  • Description

    Rabbit monoclonal [EPR22304-3] to TIM 4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, Flow Cytmore details
    Unsuitable for: IHC-P,IP or WB
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human TIM 4 aa 1-150. The exact sequence is proprietary.
    Database link: Q96H15

  • Positive control

    • ICC/IF: HEK-293T cells. Flow Cyt: HEK-293T cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR22304-3
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab222093 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.
Flow Cyt 1/500.
  • Application notes
    Is unsuitable for IHC-P,IP or WB.
  • Target

    • Function

      Phosphatidylserine receptor that enhances the engulfment of apoptotic cells. Involved in regulating T-cell proliferation and lymphotoxin signaling. Ligand for HAVCR1/TIMD1.
    • Sequence similarities

      Belongs to the immunoglobulin superfamily. TIM family.
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    • Domain

      Recognizes phosphatidyl serine via its immunoglobulin domain.
    • Cellular localization

      Membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • SMUCKLER antibody
      • Spleen, mucin-containing, knockout of lymphotoxin protein antibody
      • T cell immunoglobulin and mucin domain containing protein 4 antibody
      • T-cell immunoglobulin and mucin domain containing 4 antibody
      • T-cell immunoglobulin and mucin domain containing molecule antibody
      • T-cell immunoglobulin and mucin domain-containing protein 4 antibody
      • T-cell immunoglobulin and mucin domains-containing protein 4 antibody
      • T-cell membrane protein 4 antibody
      • TIM-4 antibody
      • Tim4 antibody
      • TIMD 4 antibody
      • TIMD-4 antibody
      • Timd4 antibody
      • TIMD4_HUMAN antibody
      see all

    Images

    • Flow cytometric analysis of 4% paraformaldehyde-fixed HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with a myc-tagged TIM 4 expression construct labeling TIM 4 with ab222093 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730, Black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody, Blue).  

      Cells were surface stained with ab222093. Then fixed with 2% PFA for 10min, followed by intracellular staining of anti-myc tag conjugated to Alexa Fluor®647.

      Gated on myc -positive population.

    • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized TIM 4 transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells labeling TIM 4 (Green) with ab222093 at 1/100 dilution, followed by AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) antibody at 1/1000 dilution. Confocal image showing positive staining in HEK-293T cells transfected with a myc-tagged TIM 4 expression construct. myc-Tag (9B11) Mouse mAb (Alexa Fluor® 594 Conjugate) (ab223894) was used as the counterstain (Red) at 1/100 dilution. The nuclear counterstain was DAPI (Blue).

      Negative control: Myc only transfected HEK-293T cells.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is AlexaFluor®488 Goat anti-Rabbit secondary (ab150077).

    References

    ab222093 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab222093.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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