Overview

  • Product name

    Anti-TIM22 antibody [EPR9973]
    See all TIM22 primary antibodies
  • Description

    Rabbit monoclonal [EPR9973] to TIM22
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human TIM22.
    Database link: Q9Y584

  • Positive control

    • A673, T47D, A431 and BxPC-3 cell lysates
  • General notes

     

     

     This product was previously labelled as TIMM22

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab167423 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 20 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
  • Target

    • Relevance

      TIMM22 is an essential core component of the TIM22 protein insertion complex, a complex that mediates the import and insertion of multi-pass transmembrane proteins into the mitochondrial inner membrane. In the TIM22 complex, it constitutes the voltage-activated, signal-gated hydrophilic high-conductance channel. It forms a twin-pore translocase that uses the membrane potential as an external driving force in 2 voltage-dependent steps.
    • Cellular localization

      Mitochondrion inner membrane; Multi-pass membrane protein.
    • Database links

    • Alternative names

      • Mitochondrial import inner membrane translocase subunit Tim22 antibody
      • Putative membrane protein antibody
      • Testis expressed sequence 4 antibody
      • TEX 4 antibody
      • TEX4 antibody
      • TIM 22 antibody
      • TIM22 antibody
      • TIMM 22 antibody
      • Translocase of inner mitochondrial membrane 22 homolog antibody
      see all

    Images

    • All lanes : Anti-TIM22 antibody [EPR9973] (ab167423) at 1/1000 dilution

      Lane 1 : A673 cell lysate
      Lane 2 : T47D cell lysate
      Lane 3 : A431 cell lysate
      Lane 4 : BxPC-3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : goat anti-rabbit HRP antibody at 1/2000 dilution

      Predicted band size: 20 kDa

    References

    This product has been referenced in:

    • Okuyama K  et al. Mieap-induced accumulation of lysosomes within mitochondria (MALM) regulates gastric cancer cell invasion under hypoxia by suppressing reactive oxygen species accumulation. Sci Rep 9:2822 (2019). Read more (PubMed: 30808977) »
    • Thompson K  et al. Recurrent De Novo Dominant Mutations in SLC25A4 Cause Severe Early-Onset Mitochondrial Disease and Loss of Mitochondrial DNA Copy Number. Am J Hum Genet 99:860-876 (2016). Read more (PubMed: 27693233) »
    See all 2 Publications for this product

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Human Tissue lysate - other (Mitochondria from cortex, human tissue)
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis-Tris gradient gel. Laemmli buffer with 10% β-Mercaptoethanol. Samples were denatured at 55C for 10 min. Immobilon-FL transfer membranes. Overnight transfer at +4C.)
    Loading amount
    15 µg
    Specification
    Mitochondria from cortex, human tissue
    Blocking step
    SEA Block Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 22°C

    Abcam user community

    Verified customer

    Submitted Aug 15 2019

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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