Anti-TIM44 antibody (ab168649)
Key features and details
- Mouse polyclonal to TIM44
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-TIM44 antibody
See all TIM44 primary antibodies -
Description
Mouse polyclonal to TIM44 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Recombinant full length protein within Human TIM44 aa 1 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. NP_006342.2
Database link: O43615 -
Positive control
- TIM44 transfected 293T cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.4
Constituent: 99% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab168649 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (2) |
Use a concentration of 1 µg/ml. Predicted molecular weight: 51 kDa.
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| Notes |
|---|
|
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 51 kDa. |
Target
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Relevance
TIM44 is the essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner. It recruits mitochondrial HSP70 to drive protein translocation into the matrix using ATP as an energy source. -
Cellular localization
Mitochondrion inner membrane. -
Database links
- Entrez Gene: 10469 Human
- Entrez Gene: 21856 Mouse
- Entrez Gene: 29635 Rat
- Omim: 605058 Human
- SwissProt: O43615 Human
- SwissProt: O35857 Mouse
- SwissProt: O35094 Rat
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Alternative names
- DKFZp686H05241 antibody
- MIMT44 antibody
- Mitochondrial inner membrane translocase antibody
see all
Images
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Western blot - Anti-TIM44 antibody (ab168649)All lanes : Anti-TIM44 antibody (ab168649) at 1 µg/ml
Lane 1 : TIMM44 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 15 µl per lane.
Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP
at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 51 kDa
Datasheets and documents
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Datasheet download
References (1)
ab168649 has been referenced in 1 publication.
- Vais H et al. EMRE Is a Matrix Ca(2+) Sensor that Governs Gatekeeping of the Mitochondrial Ca(2+) Uniporter. Cell Rep 14:403-410 (2016). PubMed: 26774479
