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    tim44-antibody-ab168649.pdf

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Metabolism Pathways and Processes Mitochondrial Metabolism Mitochondrial Biogenesis
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Anti-TIM44 antibody (ab168649)

  • Datasheet
Reviews (2) Submit a question References (1)

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Western blot - Anti-TIM44 antibody (ab168649)

    Key features and details

    • Mouse polyclonal to TIM44
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    Get better batch-to-batch reproducibility with a recombinant antibody

    Product image
    Anti-TIM44 antibody [EPR16821-26] - BSA and Azide free (ab232592)
    • Research with confidence – consistent and reproducible results with every batch
    • Long-term and scalable supply – powered by recombinant technology for fast production
    • Success from the first experiment – confirmed specificity through extensive validation
    • Ethical standards compliant – production is animal-free

    Overview

    • Product name

      Anti-TIM44 antibody
      See all TIM44 primary antibodies
    • Description

      Mouse polyclonal to TIM44
    • Host species

      Mouse
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Human
      Predicted to work with: Mouse, Rat
    • Immunogen

      Recombinant full length protein within Human TIM44 aa 1 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. NP_006342.2
      Database link: O43615

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • Positive control

      • TIM44 transfected 293T cell lysate.
    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
    • Storage buffer

      pH: 7.4
      Constituent: 99% PBS
    • Concentration information loading...
    • Purity

      Protein A purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Metabolism
      • Pathways and Processes
      • Mitochondrial Metabolism
      • Mitochondrial Biogenesis

    Associated products

    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Isotype control

      • Mouse IgG - Isotype Control (ab37355)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab168649 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB (2)
    Use a concentration of 1 µg/ml. Predicted molecular weight: 51 kDa.
    Notes
    WB
    Use a concentration of 1 µg/ml. Predicted molecular weight: 51 kDa.

    Target

    • Relevance

      TIM44 is the essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner. It recruits mitochondrial HSP70 to drive protein translocation into the matrix using ATP as an energy source.
    • Cellular localization

      Mitochondrion inner membrane.
    • Database links

      • Entrez Gene: 10469 Human
      • Entrez Gene: 21856 Mouse
      • Entrez Gene: 29635 Rat
      • Omim: 605058 Human
      • SwissProt: O43615 Human
      • SwissProt: O35857 Mouse
      • SwissProt: O35094 Rat
      • Alternative names

        • DKFZp686H05241 antibody
        • MIMT44 antibody
        • Mitochondrial inner membrane translocase antibody
        • TIM44 antibody
        • Translocase of inner mitochondrial membrane 44 antibody
        • Translocase of inner mitochondrial membrane 44 homolog (yeast) antibody
        see all

      Images

      • Western blot - Anti-TIM44 antibody (ab168649)
        Western blot - Anti-TIM44 antibody (ab168649)
        All lanes : Anti-TIM44 antibody (ab168649) at 1 µg/ml

        Lane 1 : TIMM44 transfected 293T cell lysate

        Lane 2 : Non-transfected 293T cell lysate


        Lysates/proteins at 15 µl per lane.

        Secondary
        All lanes : Goat Anti-Mouse IgG (H&L)-HRP
        at 1/2500 dilution

        Developed using the ECL technique.

        Predicted band size: 51 kDa

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet download

        Download

      References (1)

      Publishing research using ab168649? Please let us know so that we can cite the reference in this datasheet.

      ab168649 has been referenced in 1 publication.

      • Vais H  et al. EMRE Is a Matrix Ca(2+) Sensor that Governs Gatekeeping of the Mitochondrial Ca(2+) Uniporter. Cell Rep 14:403-410 (2016). PubMed: 26774479

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      1-2 of 2 Abreviews or Q&A

      Western blot abreview for Anti-TIM44 antibody

      Below Average
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Mouse Tissue lysate - whole (Skeletal muscle (Quadriceps))
      Gel Running Conditions
      Reduced Denaturing (gel 10%)
      Loading amount
      10 µg
      Specification
      Skeletal muscle (Quadriceps)
      Blocking step
      fish gelatine as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 8% · Temperature: RT°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      Submitted Aug 26 2020

      Western blot abreview for Anti-TIM44 antibody

      Poor
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Mouse Tissue lysate - other (Mouse brain homogenate, Mitochondria from mouse br)
      Gel Running Conditions
      Reduced Denaturing (4-12% SDS-PAGE, 55C for 10 min denaturation)
      Loading amount
      20 µg
      Specification
      Mouse brain homogenate, Mitochondria from mouse br
      Blocking step
      SEA Block Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 22°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Svitlana Yablonska

      Verified customer

      Submitted Jul 20 2017

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