Overview

  • Product name
    Anti-TIM44 antibody
    See all TIM44 primary antibodies
  • Description
    Mouse polyclonal to TIM44
  • Host species
    Mouse
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Full length protein corresponding to Human TIM44 aa 1-452.
    Sequence:

    MAAAALRSGWCRCPRRCLGSGIQFLSSHNLPHGSTYQMRRPGGELPLSKS YSSGNRKGFLSGLLDNVKQELAKNKEMKESIKKFRDEARRLEESDVLQEA RRKYKTIESETVRTSEVLRKKLGELTGTVKESLHEVSKSDLGRKIKEGVE EAAKTAKQSAESVSKGGEKLGRTAAFRALSQGVESVKKEIDDSVLGQTGP YRRPQRLRKRTEFAGDKFKEEKVFEPNEEALGVVLHKDSKWYQQWKDFKE NNVVFNRFFEMKMKYDESDNAFIRASRALTDKVTDLLGGLFSKTEMSEVL TEILRVDPAFDKDRFLKQCENDIIPNVLEAMISGELDILKDWCYEATYSQ LAHPIQQAKALGLQFHSRILDIDNVDLAMGKMMEQGPVLIITFQAQLVMV VRNPKGEVVEGDPDKVLRMLYVWALCRDQDELNPYAAWRLLDISASSTEQ IL

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • TIMM44 transfected 293T cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab168649 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 51 kDa.

Target

  • Relevance
    TIM44 is the essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner. It recruits mitochondrial HSP70 to drive protein translocation into the matrix using ATP as an energy source.
  • Cellular localization
    Mitochondrion inner membrane.
  • Database links
    • Alternative names
      • DKFZp686H05241 antibody
      • MIMT44 antibody
      • Mitochondrial inner membrane translocase antibody
      • TIM44 antibody
      • Translocase of inner mitochondrial membrane 44 antibody
      • Translocase of inner mitochondrial membrane 44 homolog (yeast) antibody
      see all

    Images

    • Western blot - Anti-TIM44 antibody (ab168649)
      Western blot - Anti-TIM44 antibody (ab168649)
      All lanes : Anti-TIM44 antibody (ab168649) at 1 µg/ml

      Lane 1 : TIMM44 transfected 293T cell lysate

      Lane 2 : Non-transfected 293T cell lysate


      Lysates/proteins at 15 µl per lane.

      Secondary
      All lanes : Goat Anti-Mouse IgG (H&L)-HRP
      at 1/2500 dilution

      Developed using the ECL technique.

      Predicted band size: 51 kDa

    References

    This product has been referenced in:
    • Vais H  et al. EMRE Is a Matrix Ca(2+) Sensor that Governs Gatekeeping of the Mitochondrial Ca(2+) Uniporter. Cell Rep 14:403-410 (2016). Read more (PubMed: 26774479) »

    See 1 Publication for this product

    Publishing research using ab168649? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    1 Review or Q&A

    10
    Votes: Highest First

    Western blot abreview for Anti-TIM44 antibody

     Poor
    Abreviews
    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Tissue lysate - other (Mouse brain homogenate, Mitochondria from mouse br)
    Gel Running Conditions
    Reduced Denaturing (4-12% SDS-PAGE, 55C for 10 min denaturation)
    Loading amount
    20 µg
    Specification
    Mouse brain homogenate, Mitochondria from mouse br
    Blocking step
    SEA Block Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 22°C
    Read More
    abreview image
    Username

    Svitlana Yablonska

    Verified customer

    Submitted Jul 20 2017

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