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    tim44-antibody-ab244466.pdf

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Metabolism Pathways and Processes Mitochondrial Metabolism Mitochondrial Biogenesis
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Anti-TIM44 antibody (ab244466)

  • Datasheet
  • SDS
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Western blot - Anti-TIM44 antibody (ab244466)
  • Immunocytochemistry/ Immunofluorescence - Anti-TIM44 antibody (ab244466)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIM44 antibody (ab244466)
  • Western blot - Anti-TIM44 antibody (ab244466)
  • Western blot - Anti-TIM44 antibody (ab244466)
  • Western blot - Anti-TIM44 antibody (ab244466)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIM44 antibody (ab244466)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIM44 antibody (ab244466)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIM44 antibody (ab244466)

Key features and details

  • Rabbit polyclonal to TIM44
  • Suitable for: WB, ICC/IF, IHC-P
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-TIM44 antibody
    See all TIM44 primary antibodies
  • Description

    Rabbit polyclonal to TIM44
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment corresponding to Human TIM44 aa 100-250.
    Database link: O43615

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • IHC-P: Human cerebral cortex, heart, gastrointestinal and testis tissue. WB: PC-3 and NBT-II cell lysate ICC/IF: A431 cells.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial Biogenesis

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab244466 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use a concentration of 0.04 - 0.4 µg/ml.
ICC/IF
Use a concentration of 0.25 - 2 µg/ml.

Fixation/Permeabilization: PFA/Triton X-100.

IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
Use a concentration of 0.04 - 0.4 µg/ml.
ICC/IF
Use a concentration of 0.25 - 2 µg/ml.

Fixation/Permeabilization: PFA/Triton X-100.

IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Relevance

    TIM44 is the essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner. It recruits mitochondrial HSP70 to drive protein translocation into the matrix using ATP as an energy source.
  • Cellular localization

    Mitochondrion inner membrane.
  • Database links

    • Entrez Gene: 10469 Human
    • Entrez Gene: 21856 Mouse
    • Entrez Gene: 29635 Rat
    • Omim: 605058 Human
    • SwissProt: O43615 Human
    • SwissProt: O35857 Mouse
    • SwissProt: O35094 Rat
    • Alternative names

      • DKFZp686H05241 antibody
      • MIMT44 antibody
      • Mitochondrial inner membrane translocase antibody
      • TIM44 antibody
      • Translocase of inner mitochondrial membrane 44 antibody
      • Translocase of inner mitochondrial membrane 44 homolog (yeast) antibody
      see all

    Images

    • Western blot - Anti-TIM44 antibody (ab244466)
      Western blot - Anti-TIM44 antibody (ab244466)
      All lanes : Anti-TIM44 antibody (ab244466) at 0.4 µg/ml

      Lane 1 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
      Lane 2 : NBT-II whole cell lysate
    • Immunocytochemistry/ Immunofluorescence - Anti-TIM44 antibody (ab244466)
      Immunocytochemistry/ Immunofluorescence - Anti-TIM44 antibody (ab244466)

      PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for TIM44 (green) using ab244466 at 4 μg/ml in ICC/IF.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIM44 antibody (ab244466)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIM44 antibody (ab244466)
      Paraffin-embedded human heart muscle tissue stained for TIM44 using ab244466 at 1/200 dilution in immunohistochemical analysis.
    • Western blot - Anti-TIM44 antibody (ab244466)
      Western blot - Anti-TIM44 antibody (ab244466)
      All lanes : Anti-TIM44 antibody (ab244466) at 0.4 µg/ml

      Lane 1 : PC-3 (human prostate adenocarcinoma cell line) whole cell lysate
      Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    • Western blot - Anti-TIM44 antibody (ab244466)
      Western blot - Anti-TIM44 antibody (ab244466)
      All lanes : Anti-TIM44 antibody (ab244466) at 0.4 µg/ml

      Lane 1 : U-2 OS (human bone osteosarcoma epithelial cell line) cells transfected with siRNA probe#1
      Lane 2 : U-2 OS cells transfected with siRNA probe#2
      Lane 3 : U-2 OS cells transfected with siRNA control
    • Western blot - Anti-TIM44 antibody (ab244466)
      Western blot - Anti-TIM44 antibody (ab244466)
      All lanes : Anti-TIM44 antibody (ab244466) at 0.4 µg/ml

      Lane 1 : Caco-2 (Human colorectal adenocarcinoma cell line) cells transfected with a siRNA control
      Lane 2 : Caco-2 cells transfected with a siRNA control
      Lane 3 : Caco-2 cells transfected with a siRNA probe#2
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIM44 antibody (ab244466)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIM44 antibody (ab244466)
      Paraffin-embedded human cerebral cortex tissue stained for TIM44 using ab244466 at 1/200 dilution in immunohistochemical analysis.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIM44 antibody (ab244466)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIM44 antibody (ab244466)
      Paraffin-embedded human testis tissue stained for TIM44 using ab244466 at 1/200 dilution in immunohistochemical analysis.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIM44 antibody (ab244466)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIM44 antibody (ab244466)
      Paraffin-embedded human gastrointestinal tissue stained for TIM44 using ab244466 at 1/200 dilution in immunohistochemical analysis.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (1)

    Publishing research using ab244466? Please let us know so that we can cite the reference in this datasheet.

    ab244466 has been referenced in 1 publication.

    • Sharanek A  et al. OSMR controls glioma stem cell respiration and confers resistance of glioblastoma to ionizing radiation. Nat Commun 11:4116 (2020). PubMed: 32807793

    Customer reviews and Q&As

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