Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
RecombinantRabMAb

Recombinant Anti-TIM44 antibody [EPR16821] - BSA and Azide free (ab251197)

Submit a review Submit a question
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIM44 antibody [EPR16821] - BSA and Azide free (ab251197)
  • Western blot - Anti-TIM44 antibody [EPR16821] - BSA and Azide free (ab251197)
  • Immunocytochemistry/ Immunofluorescence - Anti-TIM44 antibody [EPR16821] - BSA and Azide free (ab251197)
  • Western blot - Anti-TIM44 antibody [EPR16821] - BSA and Azide free (ab251197)
  • Immunoprecipitation - Anti-TIM44 antibody [EPR16821] - BSA and Azide free (ab251197)
  • Anti-TIM44 antibody [EPR16821] - BSA and Azide free (ab251197)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16821] to TIM44 - BSA and Azide free
  • Suitable for: WB, IP, IHC-P, ICC/IF
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Unconjugated

Overview

  • Product name

    Anti-TIM44 antibody [EPR16821] - BSA and Azide free
    See all TIM44 primary antibodies

  • Description

    Rabbit monoclonal [EPR16821] to TIM44 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IP, IHC-P, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab251197 is the carrier-free version of ab194829.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab251197 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use at an assay dependent concentration. Detects a band of approximately 45 kDa (predicted molecular weight: 51 kDa).
IP
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
Use at an assay dependent concentration.
Notes
WB
Use at an assay dependent concentration. Detects a band of approximately 45 kDa (predicted molecular weight: 51 kDa).
IP
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
Use at an assay dependent concentration.

Target

  • Relevance

    TIM44 is the essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner. It recruits mitochondrial HSP70 to drive protein translocation into the matrix using ATP as an energy source.

  • Cellular localization

    Mitochondrion inner membrane.

  • Database links

    • Alternative names

      • DKFZp686H05241 antibody
      • MIMT44 antibody
      • Mitochondrial inner membrane translocase antibody
      • TIM44 antibody
      • Translocase of inner mitochondrial membrane 44 antibody
      • Translocase of inner mitochondrial membrane 44 homolog (yeast) antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIM44 antibody [EPR16821] - BSA and Azide free (ab251197)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIM44 antibody [EPR16821] - BSA and Azide free (ab251197)
      This data was developed using ab194829, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human endometrial adenocarcinoma tissue labeling TIM44 with ab194829 at 1/100 dilution (5μg/ml). A Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution was used as secondary (ab97051). Counterstain: Hematoxylin.
      Inset image: negative control obtained using PBS instead of ab194829. Cytoplasm staining on humanendometrial adenocarcinoma was observed. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
    • Western blot - Anti-TIM44 antibody [EPR16821] - BSA and Azide free (ab251197)
      Western blot - Anti-TIM44 antibody [EPR16821] - BSA and Azide free (ab251197)
      Anti-TIM44 antibody [EPR16821] (ab194829) at 1/1000 dilution + mouse heart tissue lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 51 kDa



      This data was developed using ab194829, the same antibody clone in a different buffer formulation.

    • Immunocytochemistry/ Immunofluorescence - Anti-TIM44 antibody [EPR16821] - BSA and Azide free (ab251197)
      Immunocytochemistry/ Immunofluorescence - Anti-TIM44 antibody [EPR16821] - BSA and Azide free (ab251197)
      This data was developed using ab194829, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of A431 cells labeling TIM44 with ab194829 at 1/50 dilution. A Goat anti rabbit IgG (Alexa Fluor488) at 1/400 dilution (ab150077) was used as secondary antibody. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% triton X-100. Counterstain: DAPI. Cytoplasm staining on A431 cell line was observed.
    • Western blot - Anti-TIM44 antibody [EPR16821] - BSA and Azide free (ab251197)
      Western blot - Anti-TIM44 antibody [EPR16821] - BSA and Azide free (ab251197)
      All lanes : Anti-TIM44 antibody [EPR16821] (ab194829) at 1/5000 dilution

      Lane 1 : A431 cell lysate
      Lane 2 : HepG2 cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 51 kDa



      This data was developed using ab194829, the same antibody clone in a different buffer formulation.

    • Immunoprecipitation - Anti-TIM44 antibody [EPR16821] - BSA and Azide free (ab251197)
      Immunoprecipitation - Anti-TIM44 antibody [EPR16821] - BSA and Azide free (ab251197)
      This data was developed using ab194829, the same antibody clone in a different buffer formulation.Immunoprecipitation analysis of immunoprecipitation pellet from K562 cell lysate immunoprecipitated using ab194826 at 1/20 dilution (Lane 1). Lane 2: PBS instead of K562 cell lysate. ab194829 at 1/1000 was used for subsequent western blot detection. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 was used as secondary antibody.
    • Anti-TIM44 antibody [EPR16821] - BSA and Azide free (ab251197)
      Anti-TIM44 antibody [EPR16821] - BSA and Azide free (ab251197)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab251197? Please let us know so that we can cite the reference in this datasheet.

    ab251197 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab251197.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com