Key features and details
- Goat polyclonal to TIM50
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-TIM50 antibody
See all TIM50 primary antibodies
DescriptionGoat polyclonal to TIM50
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Dog
- WB: human heart tissue lysate.
Previously labelled as TIMM50.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab23938 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 1.5 µg/ml. Detects a band of approximately 40 kDa.
1 hour primary incubation is recommended for this product.
FunctionEssential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane. Has some phosphatase activity in vitro; however such activity may not be relevant in vivo. Isoform 2 may participate in the release of snRNPs and SMN from the Cajal body.
Tissue specificityWidely expressed. Expressed at higher level in brain, kidney and liver (at protein level).
Sequence similaritiesBelongs to the TIM50 family.
Contains 1 FCP1 homology domain.
DomainThe FCP1 homology domain does not contain the canonical D-x-D-x-[TV] active site, suggesting that it probably does not display phosphatase activity in vivo.
Cellular localizationMitochondrion inner membrane and Nucleus speckle. Nuclear and enriched in speckles with snRNPs.
- Information by UniProt
- Homolog of yeast Tim50 antibody
- Import inner membrane translocase subunit TIM 50 antibody
- Import inner membrane translocase subunit TIM50 antibody
ab23938 has been referenced in 8 publications.
- Kabashima A et al. Fibroblast growth factor receptor inhibition induces loss of matrix MCL1 and necrosis in cholangiocarcinoma. J Hepatol 68:1228-1238 (2018). WB . PubMed: 29408314
- Chen X et al. Artesunate Activates the Intrinsic Apoptosis of HCT116 Cells through the Suppression of Fatty Acid Synthesis and the NF-?B Pathway. Molecules 22:N/A (2017). PubMed: 28786914
- Abdel Malik R et al. AMP-Activated Protein Kinase a2 in Neutrophils Regulates Vascular Repair via Hypoxia-Inducible Factor-1a and a Network of Proteins Affecting Metabolism and Apoptosis. Circ Res 120:99-109 (2017). WB ; Mouse . PubMed: 27777247
- Byun J et al. CR6-interacting factor 1 is a key regulator in Aß-induced mitochondrial disruption and pathogenesis of Alzheimer's disease. Cell Death Differ 22:959-73 (2015). WB ; Mouse . PubMed: 25361083
- Baseler WA et al. Reversal of mitochondrial proteomic loss in Type 1 diabetic heart with overexpression of phospholipid hydroperoxide glutathione peroxidase. Am J Physiol Regul Integr Comp Physiol 304:R553-65 (2013). PubMed: 23408027
- Perciavalle RM et al. Anti-apoptotic MCL-1 localizes to the mitochondrial matrix and couples mitochondrial fusion to respiration. Nat Cell Biol 14:575-83 (2012). PubMed: 22544066
- Bigi A et al. Human sialidase NEU4 long and short are extrinsic proteins bound to outer mitochondrial membrane and the endoplasmic reticulum, respectively. Glycobiology 20:148-57 (2010). WB ; African green monkey . PubMed: 19797320
- Goemans CG et al. Intra-mitochondrial degradation of Tim23 curtails the survival of cells rescued from apoptosis by caspase inhibitors. Cell Death Differ 15:545-54 (2008). WB . PubMed: 18174902