Overview

  • Product name

    Anti-TIM50 antibody [EPR5784]
    See all TIM50 primary antibodies
  • Description

    Rabbit monoclonal [EPR5784] to TIM50
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human TIM50 aa 1-100. The exact sequence is proprietary.

  • Positive control

    • 293T, SH SY5Y, HepG2 and HeLa cell lysates Human kidney tissue, human liver tissue (IHC-P)
  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

    Previously labelled as TIMM50. 

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab109527 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 40 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IP.
  • Target

    • Function

      Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane. Has some phosphatase activity in vitro; however such activity may not be relevant in vivo. Isoform 2 may participate in the release of snRNPs and SMN from the Cajal body.
    • Tissue specificity

      Widely expressed. Expressed at higher level in brain, kidney and liver (at protein level).
    • Sequence similarities

      Belongs to the TIM50 family.
      Contains 1 FCP1 homology domain.
    • Domain

      The FCP1 homology domain does not contain the canonical D-x-D-x-[TV] active site, suggesting that it probably does not display phosphatase activity in vivo.
    • Cellular localization

      Mitochondrion inner membrane and Nucleus speckle. Nuclear and enriched in speckles with snRNPs.
    • Information by UniProt
    • Database links

    • Alternative names

      • Homolog of yeast Tim50 antibody
      • Import inner membrane translocase subunit TIM 50 antibody
      • Import inner membrane translocase subunit TIM50 antibody
      • Import inner membrane translocase subunit TIM50 mitochondrial antibody
      • MGC102733 antibody
      • Mitochondrial import inner membrane translocase subunit TIM50 antibody
      • TIM 50 antibody
      • TIM 50L antibody
      • TIM50 antibody
      • Tim50 like protein antibody
      • TIM50_HUMAN antibody
      • TIM50L antibody
      • TIMM 50 antibody
      • TIMM50 antibody
      • translocase of inner mitochondrial membrane 50 homolog (S. cerevisiae) antibody
      • Translocase of inner mitochondrial membrane 50 homolog (yeast) antibody
      • Translocase of inner mitochondrial membrane 50 homolog antibody
      • Translocase of inner mitochondrial membrane 50, yeast, homolog of antibody
      see all

    Images

    • All lanes : Anti-TIM50 antibody [EPR5784] (ab109527) at 1/10000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : SH SY5Y cell lysate
      Lane 3 : HepG2 cell lysate
      Lane 4 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 40 kDa

    • ab109527, at 1/50, staining TIM50 in paraffin-embedded kidney tissue by Immunohistochemistry.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • ab109527, at 1/50, staining TIM50 in paraffin-embedded liver tissue by Immunohistochemistry.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    References

    This product has been referenced in:

    • Burstein SR  et al. In vitro and in vivo studies of the ALS-FTLD protein CHCHD10 reveal novel mitochondrial topology and protein interactions. Hum Mol Genet 27:160-177 (2018). Read more (PubMed: 29112723) »
    • Lobo-Jarne T  et al. Human COX7A2L Regulates Complex III Biogenesis and Promotes Supercomplex Organization Remodeling without Affecting Mitochondrial Bioenergetics. Cell Rep 25:1786-1799.e4 (2018). Read more (PubMed: 30428348) »
    See all 2 Publications for this product

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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