Overview

  • Product name

  • Description

    Rabbit polyclonal to Timeless
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment within Human Timeless (C terminal). The exact sequence is proprietary.
    Database link: Q9UNS1

  • Positive control

    • WB: HEK-293T, A431, HeLa and HepG2 whole cell lysates; Neuro-2a, C8D30, NIH/3T3, RAW 264.7 and C2C12 whole cell extracts. IP: HEK-293T whole cell extract. ICC/IF: HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab229218 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 139 kDa.
IP 1/100 - 1/500.
ICC/IF 1/100 - 1/2000.

Target

  • Function

    Required for normal progression of S-phase. Involved in the circadian rhythm autoregulatory loop. Negatively regulates CLOCK-NPAS2/BMAL1-induced transactivation of PER1 possibly via translocation of PER1 into the nucleus. Promotes TIPIN nuclear localiZation. Involved in cell survival after DNA damage or replication stress. May be specifically required for the ATR-CHK1 pathway in the replication checkpoint induced by hydroxyurea or ultraviolet light. May also play an important role in epithelial cell morphogenesis and formation of branching tubules.
  • Tissue specificity

    Expressed in all tissues examined including brain, heart, lung, liver, skeletal muscle, kidney, placenta, pancreas, spleen, thymus and testis. Highest levels of expression in placenta, pancreas, thymus and testis.
  • Sequence similarities

    Belongs to the timeless family.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • FLJ12640 antibody
    • FLJ20714 antibody
    • hTIM antibody
    • Protein timeless homolog antibody
    • TIM antibody
    • TIM_HUMAN antibody
    • TIM1 antibody
    • Timeless antibody
    • timeless circadian clock 1 antibody
    • timeless circadian clock antibody
    • timeless homolog antibody
    • TIMELESS1 antibody
    • Tof1 homolog antibody
    see all

Images

  • All lanes : Anti-Timeless antibody (ab229218) at 1/1000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell lysate
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 139 kDa



    5% SDS-PAGE gel.

  • All lanes : Anti-Timeless antibody (ab229218) at 1/1000 dilution

    Lane 1 : Neuro-2a (mouse neuroblastoma cell line) whole cell extract
    Lane 2 : C8D30 whole cell extract
    Lane 3 : NIH/3T3 (mouse embryonic fibroblast cell line) whole cell extract
    Lane 4 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell extract
    Lane 5 : C2C12 (mouse myoblast cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 139 kDa



    5% SDS-PAGE gel.

  • 4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Timeless (green) using ab229218 at 1/2000 dilution in ICC/IF.

    Nuclear counterstain: Hoechst 33342 (blue).

  • Timeless was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract with 5 µg of ab229218. Western blot was performed from the immunoprecipitate using ab229218. Anti-Rabbit IgG was used as a secondary reagent.

    Lane 1: HEK-293T whole cell extract (Input). 
    Lane 2: Control IgG instead of ab229218 in HEK-293T whole cell extract. 
    Lane 3: ab229218 IP in HEK-293T whole cell extract. 

References

ab229218 has not yet been referenced specifically in any publications.

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