Anti-Timeless antibody (ab245639)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Timeless antibody
See all Timeless primary antibodies -
Description
Rabbit polyclonal to Timeless -
Host species
Rabbit -
Tested applications
Suitable for: IPmore details
Unsuitable for: WB -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Timeless aa 1120-1170. The exact sequence is proprietary. NP_003911.1
Database link: Q9UNS1 -
Positive control
- IP: HeLa whole cell lysate.
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General notes
ab245639 has not performed satisfactorily when used for WB of Timeless in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of Timeless.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab245639 was affinity purified using an epitope specific to Timeless immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab245639 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | Use at 2-10 µg/mg of lysate. |
Target
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Function
Required for normal progression of S-phase. Involved in the circadian rhythm autoregulatory loop. Negatively regulates CLOCK-NPAS2/BMAL1-induced transactivation of PER1 possibly via translocation of PER1 into the nucleus. Promotes TIPIN nuclear localiZation. Involved in cell survival after DNA damage or replication stress. May be specifically required for the ATR-CHK1 pathway in the replication checkpoint induced by hydroxyurea or ultraviolet light. May also play an important role in epithelial cell morphogenesis and formation of branching tubules. -
Tissue specificity
Expressed in all tissues examined including brain, heart, lung, liver, skeletal muscle, kidney, placenta, pancreas, spleen, thymus and testis. Highest levels of expression in placenta, pancreas, thymus and testis. -
Sequence similarities
Belongs to the timeless family. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 8914 Human
- Omim: 603887 Human
- SwissProt: Q9UNS1 Human
- Unigene: 118631 Human
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Alternative names
- FLJ12640 antibody
- FLJ20714 antibody
- hTIM antibody
see all
Images
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Immunoprecipitation - Anti-Timeless antibody (ab245639)Timeless was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab245639 at 6 µg/reaction. Western blot was performed from the immunoprecipitate using ab245639 at 0.4 µg/ml.
Lane 1: ab245639 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.Detection: Chemiluminescence with exposure time of 3 minutes.
Datasheets and documents
References
ab245639 has not yet been referenced specifically in any publications.
