Anti-Timeless antibody (ab72458)
Key features and details
- Rabbit polyclonal to Timeless
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Timeless antibody
See all Timeless primary antibodies -
Description
Rabbit polyclonal to Timeless -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee, Gorilla, Orangutan -
Immunogen
Synthetic peptide (Human) corresponding to a region between residues 1170 and the C-terminus (residue 1208) of Timeless. (NP_003911.1)
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab72458 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Detects a band of approximately 139, 160 kDa (predicted molecular weight: 139 kDa).
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IP |
Use at 2-5 µg/mg of lysate.
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IHC-P |
Use at an assay dependent concentration.
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Notes |
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WB
1/2000 - 1/10000. Detects a band of approximately 139, 160 kDa (predicted molecular weight: 139 kDa). |
IP
Use at 2-5 µg/mg of lysate. |
IHC-P
Use at an assay dependent concentration. |
Target
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Function
Required for normal progression of S-phase. Involved in the circadian rhythm autoregulatory loop. Negatively regulates CLOCK-NPAS2/BMAL1-induced transactivation of PER1 possibly via translocation of PER1 into the nucleus. Promotes TIPIN nuclear localiZation. Involved in cell survival after DNA damage or replication stress. May be specifically required for the ATR-CHK1 pathway in the replication checkpoint induced by hydroxyurea or ultraviolet light. May also play an important role in epithelial cell morphogenesis and formation of branching tubules. -
Tissue specificity
Expressed in all tissues examined including brain, heart, lung, liver, skeletal muscle, kidney, placenta, pancreas, spleen, thymus and testis. Highest levels of expression in placenta, pancreas, thymus and testis. -
Sequence similarities
Belongs to the timeless family. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 8914 Human
- Omim: 603887 Human
- SwissProt: Q9UNS1 Human
- Unigene: 118631 Human
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Alternative names
- FLJ12640 antibody
- FLJ20714 antibody
- hTIM antibody
see all
Images
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HeLa whole cell lysate, (1 mg for IP, 20% of IP loaded) with ab 72458 used for IP at 3 µg/mg lysate, and for subsequent WB at 0.1 µg/ml.
Detection: Chemiluminescence with an exposure time of 3 seconds. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling Timeless with ab72458 at 1/200 (1µg/ml). Detection: DAB
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling Timeless with ab72458 at 1/400 (0.5µg/ml). A DyLight® 594-conjugated goat anti-rabbit IgG (H+L) (1/100) was used as the secondary antibody.
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All lanes : Anti-Timeless antibody (ab72458) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Predicted band size: 139 kDa
Observed band size: 139 kDa
Additional bands at: 160 kDa. We are unsure as to the identity of these extra bands. -
IHC image of ab72458 staining Timeless in Human Tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab72458, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (8)
ab72458 has been referenced in 8 publications.
- Li B et al. TIMELESS inhibits breast cancer cell invasion and metastasis by down-regulating the expression of MMP9. Cancer Cell Int 21:38 (2021). PubMed: 33430865
- Mosna K et al. Complex changes of circadian proteins expression in inflammatory bowel disease. Bratisl Lek Listy 122:235-241 (2021). PubMed: 33729814
- Xing X et al. TIMELESS Promotes Tumor Progression by Enhancing Macrophages Recruitment in Ovarian Cancer. Front Oncol 11:732058 (2021). PubMed: 34490127
- Parascandolo A et al. Effect of naive and cancer-educated fibroblasts on colon cancer cell circadian growth rhythm. Cell Death Dis 11:289 (2020). PubMed: 32341349
- Cortone G et al. Interaction of the Warsaw breakage syndrome DNA helicase DDX11 with the replication fork-protection factor Timeless promotes sister chromatid cohesion. PLoS Genet 14:e1007622 (2018). PubMed: 30303954
- Calì F et al. Tim/Timeless, a member of the replication fork protection complex, operates with the Warsaw breakage syndrome DNA helicase DDX11 in the same fork recovery pathway. Nucleic Acids Res 44:705-17 (2016). IP . PubMed: 26503245
- Mazzoccoli G et al. Analysis of clock gene-miRNA correlation networks reveals candidate drivers in colorectal cancer. Oncotarget 7:45444-45461 (2016). WB . PubMed: 27323779
- Aria V et al. The human Tim-Tipin complex interacts directly with DNA polymerase epsilon and stimulates its synthetic activity. J Biol Chem 288:12742-52 (2013). IP ; Human . PubMed: 23511638