Overview

  • Product name

    Anti-Timeless antibody [EPR5275]
    See all Timeless primary antibodies
  • Description

    Rabbit monoclonal [EPR5275] to Timeless
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Timeless aa 1150 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: Q9UNS1

  • Positive control

    • HeLa, Jurkat, JAR, and BxPC-3 cell lysates, Human colon and Human tonsil tissues
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab109512 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB 1/50000 - 1/200000. Detects a band of approximately 150 kDa (predicted molecular weight: 139 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function

      Required for normal progression of S-phase. Involved in the circadian rhythm autoregulatory loop. Negatively regulates CLOCK-NPAS2/BMAL1-induced transactivation of PER1 possibly via translocation of PER1 into the nucleus. Promotes TIPIN nuclear localiZation. Involved in cell survival after DNA damage or replication stress. May be specifically required for the ATR-CHK1 pathway in the replication checkpoint induced by hydroxyurea or ultraviolet light. May also play an important role in epithelial cell morphogenesis and formation of branching tubules.
    • Tissue specificity

      Expressed in all tissues examined including brain, heart, lung, liver, skeletal muscle, kidney, placenta, pancreas, spleen, thymus and testis. Highest levels of expression in placenta, pancreas, thymus and testis.
    • Sequence similarities

      Belongs to the timeless family.
    • Cellular localization

      Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • FLJ12640 antibody
      • FLJ20714 antibody
      • hTIM antibody
      • Protein timeless homolog antibody
      • TIM antibody
      • TIM_HUMAN antibody
      • TIM1 antibody
      • Timeless antibody
      • timeless circadian clock 1 antibody
      • timeless circadian clock antibody
      • timeless homolog antibody
      • TIMELESS1 antibody
      • Tof1 homolog antibody
      see all

    Images

    • All lanes : Anti-Timeless antibody [EPR5275] (ab109512) at 1/50000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : JAR cell lysate
      Lane 4 : BxPC-3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 139 kDa
      Observed band size: 150 kDa
      why is the actual band size different from the predicted?

    • Immunocytochemistry/Immunofluorescence analysis of HT-29 (Human colorectal adenocarcinoma cell line) labeling Timeless with Purified ab109512 at 1/1000 dilution (5 µg/ml). Cells were fixed with 4% PFA and permeabilized with 0.1% tritonX-100. ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) at 1/1000 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.

    • Immunohistochemical analysis of paraffin-embedded Human colon tissue using ab109512 at a dilution of 1/100.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemical analysis of paraffin-embedded Human tonsil tissue using ab109512 at a dilution of 1/100.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    References

    This product has been referenced in:

    • Bianco JN  et al. Overexpression of Claspin and Timeless protects cancer cells from replication stress in a checkpoint-independent manner. Nat Commun 10:910 (2019). Read more (PubMed: 30796221) »
    • Baldeyron C  et al. TIPIN depletion leads to apoptosis in breast cancer cells. Mol Oncol 9:1580-98 (2015). Read more (PubMed: 26004086) »
    See all 2 Publications for this product

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