Overview

  • Product name

    Anti-Timeless antibody - N-terminal
    See all Timeless primary antibodies
  • Description

    Rabbit polyclonal to Timeless - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Cow
  • Immunogen

    Recombinant fragment within Human Timeless (N terminal). The exact sequence is proprietary.
    Database link: Q9UNS1

  • Positive control

    • WB: HeLa S3 whole cell lysate. IHC-P: SNU16 xenograft tissue. ICC/IF: HeLa cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab228599 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/1000.
IHC-P 1/100 - 1/1000.
WB 1/500 - 1/3000. Predicted molecular weight: 139 kDa.

Target

  • Function

    Required for normal progression of S-phase. Involved in the circadian rhythm autoregulatory loop. Negatively regulates CLOCK-NPAS2/BMAL1-induced transactivation of PER1 possibly via translocation of PER1 into the nucleus. Promotes TIPIN nuclear localiZation. Involved in cell survival after DNA damage or replication stress. May be specifically required for the ATR-CHK1 pathway in the replication checkpoint induced by hydroxyurea or ultraviolet light. May also play an important role in epithelial cell morphogenesis and formation of branching tubules.
  • Tissue specificity

    Expressed in all tissues examined including brain, heart, lung, liver, skeletal muscle, kidney, placenta, pancreas, spleen, thymus and testis. Highest levels of expression in placenta, pancreas, thymus and testis.
  • Sequence similarities

    Belongs to the timeless family.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • FLJ12640 antibody
    • FLJ20714 antibody
    • hTIM antibody
    • Protein timeless homolog antibody
    • TIM antibody
    • TIM_HUMAN antibody
    • TIM1 antibody
    • Timeless antibody
    • timeless circadian clock 1 antibody
    • timeless circadian clock antibody
    • timeless homolog antibody
    • TIMELESS1 antibody
    • Tof1 homolog antibody
    see all

Images

  • Anti-Timeless antibody - N-terminal (ab228599) at 1/500 dilution + HeLa S3 (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 30 µg

    Developed using the ECL technique.

    Predicted band size: 139 kDa



    7.5% SDS-PAGE

  • Paraffin-embedded SNU16 xenograft tissue stained for Timeless with ab228599 at 1/100 dilution in immunohistochemical analysis.

  • Paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Timeless (green) using ab228599 at 1/500 dilution in ICC/IF.

    Blue: DNA probe.

References

ab228599 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab228599.
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