Recombinant Anti-TIMM17A/TIM17 antibody [EPR16098] (ab192246)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16098] to TIMM17A/TIM17
- Suitable for: ICC/IF, IP, WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
-
Product name
Anti-TIMM17A/TIM17 antibody [EPR16098]
See all TIMM17A/TIM17 primary antibodies -
Description
Rabbit monoclonal [EPR16098] to TIMM17A/TIM17 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IP, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- HeLa, K562, Raw264.7, PC-12 and NIH/3T3 cell lysates; HeLa cells.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16098 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab192246 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF |
1/50.
|
|
IP |
1/40.
|
|
WB | (4) |
1/1000. Detects a band of approximately 17 kDa (predicted molecular weight: 18 kDa).
|
Notes |
---|
ICC/IF
1/50. |
IP
1/40. |
WB
1/1000. Detects a band of approximately 17 kDa (predicted molecular weight: 18 kDa). |
Target
-
Function
Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane. -
Sequence similarities
Belongs to the Tim17/Tim22/Tim23 family. -
Cellular localization
Mitochondrion inner membrane. - Information by UniProt
-
Database links
- Entrez Gene: 10440 Human
- Entrez Gene: 21854 Mouse
- Entrez Gene: 54311 Rat
- Omim: 605057 Human
- SwissProt: Q99595 Human
- SwissProt: Q9Z0V8 Mouse
- SwissProt: O35092 Rat
- Unigene: 20716 Human
see all -
Alternative names
- Inner membrane preprotein translocase Tim17a antibody
- Mitochondrial import inner membrane translocase subunit Tim17-A antibody
- mitochondrial inner membrane translocase antibody
see all
Images
-
All lanes : Anti-TIMM17A/TIM17 antibody [EPR16098] (ab192246) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : K562 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 18 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM /TBST.
-
All lanes : Anti-TIMM17A/TIM17 antibody [EPR16098] (ab192246) at 1/1000 dilution
Lane 1 : RAW 264.7 cell lysate
Lane 2 : PC-12 cell lysate
Lane 3 : NIH/3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 18 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM /TBST.
-
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized HeLa cells labeling TIMM17A/TIM17 with ab192246 at 1/50 dilution (11.7 μg/ml), followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody (ab150078) at 1/500 dilution. Nuclear counter stain Dapi (blue).
The two negative controls are ab192246 at 1/50 dilution followed by (Goat anti mouse IgG (Alexa Fluor®488) secondary antibody at 1/200 dilution.
-
Western blot analysis of TIMM17A/TIM17 in HeLa cell lysates immunoprecipitated using ab192246 at 1/40 dilution (Lane 1). Lane 2: PBS instead of HeLa lysate.
Secondary antibody: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.
Blocking/Dilution buffer: 5% NFDM /TBST.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
Certificate of Compliance
References (2)
ab192246 has been referenced in 2 publications.
- Nandi SS et al. Neurogenic Hypertension Mediated Mitochondrial Abnormality Leads to Cardiomyopathy: Contribution of UPRmt and Norepinephrine-miR- 18a-5p-HIF-1a Axis. Front Physiol 12:718982 (2021). PubMed: 34912235
- Singh T et al. Neuronal mitochondrial dysfunction in sporadic amyotrophic lateral sclerosis is developmentally regulated. Sci Rep 11:18916 (2021). PubMed: 34556702