Overview

  • Product name

    Anti-TIMM23/TIM23 antibody
    See all TIMM23/TIM23 primary antibodies
  • Description

    Rabbit polyclonal to TIMM23/TIM23
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human TIMM23/TIM23 aa 130-158 (C terminal) conjugated to keyhole limpet haemocyanin.
    Database link: O14925

  • Positive control

    • WB: MCF7 and A431 cell lysate.
  • General notes

     This product was previously labelled as TIMM23

     

Properties

Applications

Our Abpromise guarantee covers the use of ab116329 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 22 kDa.

Target

  • Relevance

    TIMM23 belongs to the Tim17/Tim22/Tim23 family and is an essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane.
  • Cellular localization

    Mitochondrion inner membrane; Multi-pass membrane protein.
  • Database links

  • Alternative names

    • MGC22767 antibody
    • MGC93478 antibody
    • Mitochondrial import inner membrane translocase subunit Tim23 antibody
    • Predicted protein of HQ1197 antibody
    • PRO1197 antibody
    • TIM23 antibody
    • TIMM 23 antibody
    • TIMM23 antibody
    • Translocase of inner mitochondrial membrane 23 (yeast) homolog antibody
    • Translocase of inner mitochondrial membrane 23 homolog (yeast) antibody
    • Translocase of inner mitochondrial membrane 23 homolog B antibody
    • Translocation of mitochondrial precursor proteins antibody
    see all

Images

  • All lanes : Anti-TIMM23/TIM23 antibody (ab116329) at 1/1000 dilution

    Lane 1 : MCF-7 cell lysate
    Lane 2 : A431 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit IgG (H+L) at 1/10000 dilution

    Predicted band size: 22 kDa

  • Western blot analysis of NB4 human cell line (cell fractionation, 30µg/lane) labelling TIMM23 with ab116329 at 1/500.  ab97051 was used as the secondary antibody (1/10000).

    Treatment - DMSO Control (CTL), 0,5 mM Hydroxyurea (HU), 1 µM Doxorubicin (DR).

    See Abreview

References

This product has been referenced in:

  • Paudel I  et al. Sab concentrations indicate chemotherapeutic susceptibility in ovarian cancer cell lines. Biochem J 475:3471-3492 (2018). Read more (PubMed: 30322886) »
  • Wai T  et al. The membrane scaffold SLP2 anchors a proteolytic hub in mitochondria containing PARL and the i-AAA protease YME1L. EMBO Rep 17:1844-1856 (2016). Read more (PubMed: 27737933) »
See all 5 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Western blot
Sample
Human Cell lysate - other (human fibroblast cell and mitochondria fraction is)
Gel Running Conditions
Reduced Denaturing
Loading amount
100 µg
Specification
human fibroblast cell and mitochondria fraction is
Blocking step
BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Aug 09 2017

Application
Western blot
Sample
Human Cell lysate - other (NB4 human cell line, cell fractionation)
Gel Running Conditions
Reduced Denaturing (13%)
Loading amount
30 µg
Treatment
DMSO Control (CTL), 0,5 mM Hydroxyurea (HU), 1 µM Doxorubicin (DR)
Specification
NB4 human cell line, cell fractionation
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Mr. Christian Marx

Verified customer

Submitted Feb 15 2013

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