Key features and details
- Rabbit polyclonal to TIMM23/TIM23
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-TIMM23/TIM23 antibody
DescriptionRabbit polyclonal to TIMM23/TIM23
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Cow, Orangutan
Recombinant full length protein corresponding to Human TIMM23/TIM23 aa 1-209.
MEGGGGSGNKTTGGLAGFFGAGGAGYSHADLAGVPLTGMNPLSPYLNVDP RYLVQDTDEFILPTGANKTRGRFELAFFTIGGCCMTGAAFGAMNGLRLGL KETQNMAWSKPRNVQILNMVTRQGALWANTLGSLALLYSAFGVIIEKTRG AEDDLNTVAAGTMTGMLYKCTGGLRGIARGGLTGLTLTSLYALYNNWEHM KGSLLQQSL
Database link: O14925
- WB: Mouse heart and liver tissue lysates; A431, MCF7 and Jurkat whole cell lysate (ab7899). IHC-P: Human testis tissue and ovarian cancer tissue.
This product was previously labelled as TIMM23
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab230253 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
|WB||1/1000 - 1/5000. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).|
RelevanceTIMM23 belongs to the Tim17/Tim22/Tim23 family and is an essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane.
Cellular localizationMitochondrion inner membrane; Multi-pass membrane protein.
- MGC22767 antibody
- MGC93478 antibody
- Mitochondrial import inner membrane translocase subunit Tim23 antibody
All lanes : Anti-TIMM23/TIM23 antibody (ab230253) at 1/1000 dilution
Lane 1 : Mouse heart tissue lysate
Lane 2 : Mouse liver tissue lysate
Lane 3 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lane 4 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lane 5 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 22 kDa
Observed band size: 22 kDa
Paraffin-embedded human testis tissue stained for TIMM23/TIM23 with ab230253 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human ovarian cancer tissue stained for TIMM23/TIM23 with ab230253 at 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab230253 has been referenced in 1 publication.
- Gao Y et al. Role of Parkin-mediated mitophagy in the protective effect of polydatin in sepsis-induced acute kidney injury. J Transl Med 18:114 (2020). PubMed: 32131850