Product nameAnti-TIMM23/TIM23 antibody
See all TIMM23/TIM23 primary antibodies
DescriptionRabbit polyclonal to TIMM23/TIM23
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Cow, Orangutan
Recombinant full length protein corresponding to Human TIMM23/TIM23 aa 1-209.
MEGGGGSGNKTTGGLAGFFGAGGAGYSHADLAGVPLTGMNPLSPYLNVDP RYLVQDTDEFILPTGANKTRGRFELAFFTIGGCCMTGAAFGAMNGLRLGL KETQNMAWSKPRNVQILNMVTRQGALWANTLGSLALLYSAFGVIIEKTRG AEDDLNTVAAGTMTGMLYKCTGGLRGIARGGLTGLTLTSLYALYNNWEHM KGSLLQQSL
Database link: O14925
- WB: Mouse heart and liver tissue lysates; A431, MCF7 and Jurkat whole cell lysate (ab7899). IHC-P: Human testis tissue and ovarian cancer tissue.
This product was previously labelled as TIMM23
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
- Mouse liver tissue lysate - total protein (ab29301)
- MCF7 whole cell lysate (ab29537)
- Jurkat whole cell lysate (ab30128)
- A431 whole cell lysate (ab30132)
- Mouse heart normal tissue lysate - total protein (ab30291)
- MCF7 whole cell lysate (ab3871)
- Jurkat whole cell lysate (ab7899)
- A431 whole cell lysate (ab7909)
Our Abpromise guarantee covers the use of ab230253 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
|WB||1/1000 - 1/5000. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).|
RelevanceTIMM23 belongs to the Tim17/Tim22/Tim23 family and is an essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane.
Cellular localizationMitochondrion inner membrane; Multi-pass membrane protein.
- MGC22767 antibody
- MGC93478 antibody
- Mitochondrial import inner membrane translocase subunit Tim23 antibody
All lanes : Anti-TIMM23/TIM23 antibody (ab230253) at 1/1000 dilution
Lane 1 : Mouse heart tissue lysate
Lane 2 : Mouse liver tissue lysate
Lane 3 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lane 4 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lane 5 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 22 kDa
Observed band size: 22 kDa
Paraffin-embedded human testis tissue stained for TIMM23/TIM23 with ab230253 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human ovarian cancer tissue stained for TIMM23/TIM23 with ab230253 at 1/100 dilution in immunohistochemical analysis.
ab230253 has not yet been referenced specifically in any publications.