Anti-TIM50 antibody (ab23938)
Key features and details
- Goat polyclonal to TIM50
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TIM50 antibody
See all TIM50 primary antibodies -
Description
Goat polyclonal to TIM50 -
Host species
Goat -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Dog -
Immunogen
Synthetic peptide corresponding to Human TIM50 aa 293-306.
Sequence:(C)DVRTVLEHYALEDD
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Positive control
- WB: human heart tissue lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab23938 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (3) |
Use a concentration of 0.5 - 1.5 µg/ml. Detects a band of approximately 40 kDa.
1 hour primary incubation is recommended for this product. |
Notes |
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WB
Use a concentration of 0.5 - 1.5 µg/ml. Detects a band of approximately 40 kDa. 1 hour primary incubation is recommended for this product. |
Target
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Function
Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane. Has some phosphatase activity in vitro; however such activity may not be relevant in vivo. Isoform 2 may participate in the release of snRNPs and SMN from the Cajal body. -
Tissue specificity
Widely expressed. Expressed at higher level in brain, kidney and liver (at protein level). -
Sequence similarities
Belongs to the TIM50 family.
Contains 1 FCP1 homology domain. -
Domain
The FCP1 homology domain does not contain the canonical D-x-D-x-[TV] active site, suggesting that it probably does not display phosphatase activity in vivo. -
Cellular localization
Mitochondrion inner membrane and Nucleus speckle. Nuclear and enriched in speckles with snRNPs. - Information by UniProt
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Database links
- Entrez Gene: 505489 Cow
- Entrez Gene: 92609 Human
- Entrez Gene: 66525 Mouse
- Entrez Gene: 685725 Rat
- Omim: 607381 Human
- SwissProt: Q3SZB3 Cow
- SwissProt: Q3ZCQ8 Human
- SwissProt: Q9D880 Mouse
see all -
Alternative names
- Homolog of yeast Tim50 antibody
- Import inner membrane translocase subunit TIM 50 antibody
- Import inner membrane translocase subunit TIM50 antibody
see all
Datasheets and documents
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SDS download
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Datasheet download
References (9)
ab23938 has been referenced in 9 publications.
- Farouk SM et al. Outer and inner mitochondrial membrane proteins TOMM40 and TIMM50 are intensively concentrated and localized at Purkinje and pyramidal neurons in the New Zealand white rabbit brain. Anat Rec (Hoboken) 305:209-221 (2022). PubMed: 34041863
- Kabashima A et al. Fibroblast growth factor receptor inhibition induces loss of matrix MCL1 and necrosis in cholangiocarcinoma. J Hepatol 68:1228-1238 (2018). WB . PubMed: 29408314
- Chen X et al. Artesunate Activates the Intrinsic Apoptosis of HCT116 Cells through the Suppression of Fatty Acid Synthesis and the NF-?B Pathway. Molecules 22:N/A (2017). PubMed: 28786914
- Abdel Malik R et al. AMP-Activated Protein Kinase a2 in Neutrophils Regulates Vascular Repair via Hypoxia-Inducible Factor-1a and a Network of Proteins Affecting Metabolism and Apoptosis. Circ Res 120:99-109 (2017). WB ; Mouse . PubMed: 27777247
- Byun J et al. CR6-interacting factor 1 is a key regulator in Aß-induced mitochondrial disruption and pathogenesis of Alzheimer's disease. Cell Death Differ 22:959-73 (2015). WB ; Mouse . PubMed: 25361083
- Baseler WA et al. Reversal of mitochondrial proteomic loss in Type 1 diabetic heart with overexpression of phospholipid hydroperoxide glutathione peroxidase. Am J Physiol Regul Integr Comp Physiol 304:R553-65 (2013). PubMed: 23408027
- Perciavalle RM et al. Anti-apoptotic MCL-1 localizes to the mitochondrial matrix and couples mitochondrial fusion to respiration. Nat Cell Biol 14:575-83 (2012). PubMed: 22544066
- Bigi A et al. Human sialidase NEU4 long and short are extrinsic proteins bound to outer mitochondrial membrane and the endoplasmic reticulum, respectively. Glycobiology 20:148-57 (2010). WB ; Human . PubMed: 19797320
- Goemans CG et al. Intra-mitochondrial degradation of Tim23 curtails the survival of cells rescued from apoptosis by caspase inhibitors. Cell Death Differ 15:545-54 (2008). WB . PubMed: 18174902