Recombinant Anti-TIM50 antibody [EPR5785] (ab109436)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5785] to TIM50
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-TIM50 antibody [EPR5785]
See all TIM50 primary antibodies -
Description
Rabbit monoclonal [EPR5785] to TIM50 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- 293T, SH-SY5Y, HepG2, and HeLa whole cell lysate (ab150035)
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Dissociation constant (KD)
KD = 1.56 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5785 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109436 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 40 kDa.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 40 kDa. |
Target
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Function
Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane. Has some phosphatase activity in vitro; however such activity may not be relevant in vivo. Isoform 2 may participate in the release of snRNPs and SMN from the Cajal body. -
Tissue specificity
Widely expressed. Expressed at higher level in brain, kidney and liver (at protein level). -
Sequence similarities
Belongs to the TIM50 family.
Contains 1 FCP1 homology domain. -
Domain
The FCP1 homology domain does not contain the canonical D-x-D-x-[TV] active site, suggesting that it probably does not display phosphatase activity in vivo. -
Cellular localization
Mitochondrion inner membrane and Nucleus speckle. Nuclear and enriched in speckles with snRNPs. - Information by UniProt
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Database links
- Entrez Gene: 92609 Human
- Entrez Gene: 66525 Mouse
- Entrez Gene: 685725 Rat
- Omim: 607381 Human
- SwissProt: Q3ZCQ8 Human
- SwissProt: Q9D880 Mouse
- Unigene: 590956 Human
- Unigene: 597106 Human
see all -
Alternative names
- Homolog of yeast Tim50 antibody
- Import inner membrane translocase subunit TIM 50 antibody
- Import inner membrane translocase subunit TIM50 antibody
see all
Images
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All lanes : Anti-TIM50 antibody [EPR5785] (ab109436) at 1/1000 dilution
Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate
Lane 3 : Hep G2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 4 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 40 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab109436 has been referenced in 5 publications.
- Katsouda A et al. MPST sulfurtransferase maintains mitochondrial protein import and cellular bioenergetics to attenuate obesity. J Exp Med 219:N/A (2022). PubMed: 35616614
- Arumugam P et al. The mitochondrial gene-CMPK2 functions as a rheostat for macrophage homeostasis. Front Immunol 13:935710 (2022). PubMed: 36451821
- Huang X et al. CHCHD2 accumulates in distressed mitochondria and facilitates oligomerization of CHCHD10. Hum Mol Genet 27:3881-3900 (2018). PubMed: 30084972
- Mon EE et al. Regulation of mitochondrial iron homeostasis by sideroflexin 2. J Physiol Sci N/A:N/A (2018). PubMed: 30570704
- Yano H et al. Inhibition of mitochondrial protein import by mutant huntingtin. Nat Neurosci 17:822-31 (2014). Mouse . PubMed: 24836077