Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11807] to TIMMDC1/C3orf1
- Suitable for: WB, ICC/IF, IP
- Reacts with: Human
Product nameAnti-TIMMDC1/C3orf1 antibody [EPR11807]
See all TIMMDC1/C3orf1 primary antibodies
DescriptionRabbit monoclonal [EPR11807] to TIMMDC1/C3orf1
Tested applicationsSuitable for: WB, ICC/IF, IPmore details
Unsuitable for: Flow Cyt or IHC-P
Species reactivityReacts with: Human
Does not react with: Mouse, Rat
Synthetic peptide within Human TIMMDC1/C3orf1 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
Database link: Q9NPL8
- THP1, U937, A375, HepG2 whole cell lysate (ab7900) and Human fetal heart tissue lysates; HepG2 cells
This product was previously labelled as TIMMDC1
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab171978 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Predicted molecular weight: 32 kDa.|
|ICC/IF||1/100 - 1/250.|
|IP||1/10 - 1/100.|
Tissue specificityGeneralized expression enhanced in heart and skeletal muscle.
Sequence similaritiesBelongs to the Tim17/Tim22/Tim23 family.
- Information by UniProt
- C3orf1 antibody
- M5 14 protein antibody
- Protein M5-14 antibody
All lanes : Anti-TIMMDC1/C3orf1 antibody [EPR11807] (ab171978) at 1/10000 dilution
Lane 1 : THP1 cell lysate
Lane 2 : U937 cell lysate
Lane 3 : A375 cell lysate
Lane 4 : HepG2 cell lysate
Lane 5 : Fetal heart tissue lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 32 kDa
Western blot analysis on immunoprecipitation pellet from A375 cell lysate labeling TIMMDC1/C3orf1 with ab171978 at 1/10 dilution.
Immunofluorescent analysis of HepG2 cells labeling TIMMDC1/C3orf1 with ab171978 at 1/100 dilution (red). DAPI nuclear staining (blue).
ab171978 has been referenced in 2 publications.
- Fan M et al. Long non-coding RNA TCF7 contributes to the growth and migration of airway smooth muscle cells in asthma through targeting TIMMDC1/Akt axis. Biochem Biophys Res Commun 508:749-755 (2019). PubMed: 30528236
- Kremer LS et al. Genetic diagnosis of Mendelian disorders via RNA sequencing. Nat Commun 8:15824 (2017). WB ; Human . PubMed: 28604674