Key features and details
- Rabbit polyclonal to TIMP1 (Biotin)
- Suitable for: WB, Sandwich ELISA
- Reacts with: Human
- Conjugation: Biotin
- Isotype: IgG
Product nameAnti-TIMP1 antibody (Biotin)
See all TIMP1 primary antibodies
DescriptionRabbit polyclonal to TIMP1 (Biotin)
Tested applicationsSuitable for: WB, Sandwich ELISAmore details
Species reactivityReacts with: Human
Highly pure (>98%) recombinant human TIMP1.
FormLyophilized:Centrifuge vial prior to opening. Reconstitute in distilled water at a concentration of 0.1-1.0 mg/ml.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab77848 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 23 kDa. Used in conjunction with compatible secondary reagents the detection limit for recombinant human TIMP1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.|
|Sandwich ELISA||Use a concentration of 0.25 - 1 µg/ml. Can be used as detection antibody with recommended pair. Use 100 µl/well antibody solution.|
FunctionComplexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Also mediates erythropoiesis in vitro; but, unlike IL-3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-11, MMP-12, MMP-13 and MMP-16. Does not act on MMP-14.
Sequence similaritiesBelongs to the protease inhibitor I35 (TIMP) family.
Contains 1 NTR domain.
modificationsThe activity of TIMP1 is dependent on the presence of disulfide bonds.
- Information by UniProt
- Clgi antibody
- Collagenase inhibitor antibody
- Collagenase inhibitor, Human antibody
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab77848 has not yet been referenced specifically in any publications.