Recombinant Anti-TIMP1 antibody [EP1549RY] (ab81282)
![Western blot - Anti-TIMP1 antibody [EP1549RY] (ab81282)](https://www.abcam.com/ps/products/81/ab81282/Images/ab81282-392500-anti-timp1-antibody-ep1549ry-westernblot-timp1-recombinant-protein-human.jpg "Western blot - Anti-TIMP1 antibody [EP1549RY] (ab81282)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1549RY] to TIMP1
- Suitable for: WB
- Reacts with: Human, Recombinant fragment
Overview
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Product name
Anti-TIMP1 antibody [EP1549RY]
See all TIMP1 primary antibodies -
Description
Rabbit monoclonal [EP1549RY] to TIMP1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human, Recombinant fragment -
Immunogen
Synthetic peptide within Human TIMP1 aa 150-250 (C terminal). The exact sequence is proprietary.
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Positive control
- WB: Human TIMP1 recombinant protein
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1549RY -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab81282 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Predicted molecular weight: 23 kDa.
For unpurified use at 1/100 - 1/500. |
| Notes |
|---|
|
WB
1/1000. Predicted molecular weight: 23 kDa. For unpurified use at 1/100 - 1/500. |
Target
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Function
Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Also mediates erythropoiesis in vitro; but, unlike IL-3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-11, MMP-12, MMP-13 and MMP-16. Does not act on MMP-14. -
Sequence similarities
Belongs to the protease inhibitor I35 (TIMP) family.
Contains 1 NTR domain. -
Post-translational
modificationsThe activity of TIMP1 is dependent on the presence of disulfide bonds. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 7076 Human
- Omim: 305370 Human
- SwissProt: P01033 Human
- Unigene: 522632 Human
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Alternative names
- Clgi antibody
- Collagenase inhibitor antibody
- Collagenase inhibitor, Human antibody
see all
Images
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Western blot - Anti-TIMP1 antibody [EP1549RY] (ab81282)Anti-TIMP1 antibody [EP1549RY] (ab81282) at 1/1000 dilution (Purified) + Human Timp1 recombinant protein at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 23 kDa
Observed band size: 32 kDa why is the actual band size different from the predicted?Blocking/Diluting buffer: 5% NFDM/TBST
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![Western blot - Anti-TIMP1 antibody [EP1549RY] (ab81282)](https://www.abcam.com/ps/products/81/ab81282/Images/ab81282-96466-anti-timp1-antibody-ep1549ry-western-blot.jpg)
Western blot - Anti-TIMP1 antibody [EP1549RY] (ab81282)Anti-TIMP1 antibody [EP1549RY] (ab81282) at 1/500 dilution (unpurified) + 5 ng of recombinant protein
Secondary
HRP labelled goat anti rabbit at 1/2000 dilution
Predicted band size: 23 kDa
Observed band size: 32 kDa why is the actual band size different from the predicted? -
![Anti-TIMP1 antibody [EP1549RY] (ab81282)](https://www.abcam.com/ps/products/81/ab81282/Images/ab81282-3-benefits-of-recombinant-antibodies.png)
Anti-TIMP1 antibody [EP1549RY] (ab81282)
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab81282 has been referenced in 1 publication.
- Pingitore P et al. PNPLA3 overexpression results in reduction of proteins predisposing to fibrosis. Hum Mol Genet 25:5212-5222 (2016). PubMed: 27742777