Recombinant
RabMAb

Anti-TIMP1 antibody [EP1549RY] (ab81282)

Abreviews (1) Submit a Question

Overview

  • Product name
    Anti-TIMP1 antibody [EP1549RY]
    See all TIMP1 primary antibodies
  • Description
    Rabbit monoclonal [EP1549RY] to TIMP1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues in the C-terminal region of human TIMP1 was used as immunogen.

  • Positive control
    • recombinant protein
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab81282 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 23 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC,IHC-P or IP.

    • Target

    • Function
      Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Also mediates erythropoiesis in vitro; but, unlike IL-3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-11, MMP-12, MMP-13 and MMP-16. Does not act on MMP-14.
    • Sequence similarities
      Belongs to the protease inhibitor I35 (TIMP) family.
      Contains 1 NTR domain.
    • Post-translational
      modifications
      The activity of TIMP1 is dependent on the presence of disulfide bonds.
    • Cellular localization
      Secreted.
    • Information by UniProt
    • Database links
      • Alternative names
        • Clgi antibody
        • Collagenase inhibitor antibody
        • Collagenase inhibitor, Human antibody
        • EPA antibody
        • EPO antibody
        • Erythroid Potentiating Activity antibody
        • Erythroid-potentiating activity antibody
        • Fibroblast collagenase inhibitor antibody
        • FLJ90373 antibody
        • HCI antibody
        • Human Collagenase Inhibitor antibody
        • Metalloproteinase inhibitor 1 antibody
        • Metalloproteinase inhibitor 1 precursor antibody
        • OTTHUMP00000023214 antibody
        • TIMP 1 antibody
        • TIMP antibody
        • TIMP metallopeptidase inhibitor 1 antibody
        • TIMP-1 antibody
        • Timp1 antibody
        • TIMP1 protein antibody
        • TIMP1_HUMAN antibody
        • Tissue Inhibitor of Metalloproteinase 1 antibody
        • Tissue inhibitor of metalloproteinases 1 antibody
        • Tissue inhibitor of metalloproteinases antibody
        • Ttissue inhibitor of metalloproteinase 1 erythroid potentiating activity collagenase inhibitor antibody
        see all

      Images

      • Western blot - Anti-TIMP1 antibody [EP1549RY] (ab81282)
        Western blot - Anti-TIMP1 antibody [EP1549RY] (ab81282)
        Anti-TIMP1 antibody [EP1549RY] (ab81282) at 1/500 dilution + 5 ng of recombinant protein

        Secondary
        HRP labelled goat anti rabbit at 1/2000 dilution

        Predicted band size: 23 kDa
        Observed band size: 32 kDa (why is the actual band size different from the predicted?)

      References

      ab81282 has not yet been referenced specifically in any publications.

      Publishing research using ab81282? Please let us know so that we can cite the reference in this datasheet.

      Customer reviews and Q&As

      Submit a review Submit a question

      1 Review or Q&A

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      Votes: Highest First

      Immunohistochemistry (Frozen sections) abreview for Anti-TIMP1 antibody [EP1549RY]

       Good
      Abreviews
      Application
      Immunohistochemistry (Frozen sections)
      Blocking step
      0.3% Triton X-100, 10mg/mL BSA, 5% Normal Goat Serum, in PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
      Sample
      Dog Tissue sections (Left Ventricle)
      Specification
      Left Ventricle
      Permeabilization
      Yes - 0.3% Triton X-100, 10mg/mL BSA, 5% Normal Goat Serum, in PBS
      Fixative
      Paraformaldehyde
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      Submitted Aug 29 2014

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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