Recombinant
RabMAb

Recombinant Anti-TIMP1 antibody [EP1549RY] (ab81282)

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Overview

  • Product name

    Anti-TIMP1 antibody [EP1549RY]
    See all TIMP1 primary antibodies

  • Description

    Rabbit monoclonal [EP1549RY] to TIMP1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    A synthetic peptide corresponding to residues in the C-terminal region of human TIMP1 was used as immunogen.

  • Positive control

    • recombinant protein

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab81282 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 23 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC,IHC-P or IP.

    • Target

    • Function

      Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Also mediates erythropoiesis in vitro; but, unlike IL-3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-11, MMP-12, MMP-13 and MMP-16. Does not act on MMP-14.

    • Sequence similarities

      Belongs to the protease inhibitor I35 (TIMP) family.
      Contains 1 NTR domain.

    • Post-translational
      modifications

      The activity of TIMP1 is dependent on the presence of disulfide bonds.

    • Cellular localization

      Secreted.
    • Information by UniProt

    • Database links

      • Alternative names

        • Clgi antibody
        • Collagenase inhibitor antibody
        • Collagenase inhibitor, Human antibody
        • EPA antibody
        • EPO antibody
        • Erythroid Potentiating Activity antibody
        • Erythroid-potentiating activity antibody
        • Fibroblast collagenase inhibitor antibody
        • FLJ90373 antibody
        • HCI antibody
        • Human Collagenase Inhibitor antibody
        • Metalloproteinase inhibitor 1 antibody
        • Metalloproteinase inhibitor 1 precursor antibody
        • OTTHUMP00000023214 antibody
        • TIMP 1 antibody
        • TIMP antibody
        • TIMP metallopeptidase inhibitor 1 antibody
        • TIMP-1 antibody
        • Timp1 antibody
        • TIMP1 protein antibody
        • TIMP1_HUMAN antibody
        • Tissue Inhibitor of Metalloproteinase 1 antibody
        • Tissue inhibitor of metalloproteinases 1 antibody
        • Tissue inhibitor of metalloproteinases antibody
        • Ttissue inhibitor of metalloproteinase 1 erythroid potentiating activity collagenase inhibitor antibody
        see all

      Images

      • Western blot - Anti-TIMP1 antibody [EP1549RY] (ab81282)
        Western blot - Anti-TIMP1 antibody [EP1549RY] (ab81282)
        Anti-TIMP1 antibody [EP1549RY] (ab81282) at 1/500 dilution + 5 ng of recombinant protein

        Secondary
        HRP labelled goat anti rabbit at 1/2000 dilution

        Predicted band size: 23 kDa
        Observed band size: 32 kDa
        why is the actual band size different from the predicted?

      References

      This product has been referenced in:

      • Pingitore P  et al. PNPLA3 overexpression results in reduction of proteins predisposing to fibrosis. Hum Mol Genet 25:5212-5222 (2016). Read more (PubMed: 27742777) »
      See 1 Publication for this product

      Publishing research using ab81282? Please let us know so that we can cite the reference in this datasheet.

      Customer reviews and Q&As

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      Immunohistochemistry (Frozen sections) abreview for Anti-TIMP1 antibody [EP1549RY]

       Good
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Frozen sections)
      Blocking step
      0.3% Triton X-100, 10mg/mL BSA, 5% Normal Goat Serum, in PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
      Sample
      Dog Tissue sections (Left Ventricle)
      Specification
      Left Ventricle
      Permeabilization
      Yes - 0.3% Triton X-100, 10mg/mL BSA, 5% Normal Goat Serum, in PBS
      Fixative
      Paraformaldehyde
      Read More

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      Verified customer

      Submitted Aug 29 2014

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