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Cardiovascular Angiogenesis Adhesion / ECM Matrix Metalloproteinases TIMP
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RecombinantRabMAb

Recombinant Anti-TIMP1 antibody [EP1549RY] (ab81282)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (1)

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Western blot - Anti-TIMP1 antibody [EP1549RY] (ab81282)
  • Western blot - Anti-TIMP1 antibody [EP1549RY] (ab81282)
  • Anti-TIMP1 antibody [EP1549RY] (ab81282)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1549RY] to TIMP1
  • Suitable for: WB
  • Reacts with: Human, Recombinant fragment

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Protein
Product image
Recombinant human TIMP1 protein (ab82104)

View more associated products

Overview

  • Product name

    Anti-TIMP1 antibody [EP1549RY]
    See all TIMP1 primary antibodies
  • Description

    Rabbit monoclonal [EP1549RY] to TIMP1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human, Recombinant fragment
  • Immunogen

    Synthetic peptide within Human TIMP1 aa 150-250 (C terminal). The exact sequence is proprietary.

  • Positive control

    • WB: Human TIMP1 recombinant protein
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP1549RY
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Angiogenesis
    • Adhesion / ECM
    • Matrix Metalloproteinases
    • TIMP
    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • ECM Enzymes
    • TIMP1 / TIMP2
    • Cancer
    • Invasion/microenvironment
    • Angiogenesis
    • ECM enzymes
    • TIMPs
    • Cancer
    • Invasion/microenvironment
    • ECM
    • Extracellular matrix
    • TIMPs
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Protease inhibitors
    • Metalloprotease inhibitors
    • TIMPs

Associated products

  • Alternative Versions

    • Anti-TIMP1 antibody [EP1549RY] - BSA and Azide free (ab215359)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant human TIMP1 protein (ab82104)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab81282 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000. Predicted molecular weight: 23 kDa.

For unpurified use at 1/100 - 1/500.

Notes
WB
1/1000. Predicted molecular weight: 23 kDa.

For unpurified use at 1/100 - 1/500.

Target

  • Function

    Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Also mediates erythropoiesis in vitro; but, unlike IL-3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-11, MMP-12, MMP-13 and MMP-16. Does not act on MMP-14.
  • Sequence similarities

    Belongs to the protease inhibitor I35 (TIMP) family.
    Contains 1 NTR domain.
  • Post-translational
    modifications

    The activity of TIMP1 is dependent on the presence of disulfide bonds.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession P01033 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 7076 Human
    • Omim: 305370 Human
    • SwissProt: P01033 Human
    • Unigene: 522632 Human
    • Alternative names

      • Clgi antibody
      • Collagenase inhibitor antibody
      • Collagenase inhibitor, Human antibody
      • EPA antibody
      • EPO antibody
      • Erythroid Potentiating Activity antibody
      • Erythroid-potentiating activity antibody
      • Fibroblast collagenase inhibitor antibody
      • FLJ90373 antibody
      • HCI antibody
      • Human Collagenase Inhibitor antibody
      • Metalloproteinase inhibitor 1 antibody
      • Metalloproteinase inhibitor 1 precursor antibody
      • OTTHUMP00000023214 antibody
      • TIMP 1 antibody
      • TIMP antibody
      • TIMP metallopeptidase inhibitor 1 antibody
      • TIMP-1 antibody
      • Timp1 antibody
      • TIMP1 protein antibody
      • TIMP1_HUMAN antibody
      • Tissue Inhibitor of Metalloproteinase 1 antibody
      • Tissue inhibitor of metalloproteinases 1 antibody
      • Tissue inhibitor of metalloproteinases antibody
      • Ttissue inhibitor of metalloproteinase 1 erythroid potentiating activity collagenase inhibitor antibody
      see all

    Images

    • Western blot - Anti-TIMP1 antibody [EP1549RY] (ab81282)
      Western blot - Anti-TIMP1 antibody [EP1549RY] (ab81282)
      Anti-TIMP1 antibody [EP1549RY] (ab81282) at 1/1000 dilution (Purified) + Human Timp1 recombinant protein at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 23 kDa
      Observed band size: 32 kDa why is the actual band size different from the predicted?



      Blocking/Diluting buffer: 5% NFDM/TBST

    • Western blot - Anti-TIMP1 antibody [EP1549RY] (ab81282)
      Western blot - Anti-TIMP1 antibody [EP1549RY] (ab81282)
      Anti-TIMP1 antibody [EP1549RY] (ab81282) at 1/500 dilution (unpurified) + 5 ng of recombinant protein

      Secondary
      HRP labelled goat anti rabbit at 1/2000 dilution

      Predicted band size: 23 kDa
      Observed band size: 32 kDa why is the actual band size different from the predicted?

    • Anti-TIMP1 antibody [EP1549RY] (ab81282)
      Anti-TIMP1 antibody [EP1549RY] (ab81282)

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (1)

    Publishing research using ab81282? Please let us know so that we can cite the reference in this datasheet.

    ab81282 has been referenced in 1 publication.

    • Pingitore P  et al. PNPLA3 overexpression results in reduction of proteins predisposing to fibrosis. Hum Mol Genet 25:5212-5222 (2016). PubMed: 27742777

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Immunohistochemistry (Frozen sections) abreview for Anti-TIMP1 antibody [EP1549RY]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    0.3% Triton X-100, 10mg/mL BSA, 5% Normal Goat Serum, in PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Sample
    Dog Tissue sections (Left Ventricle)
    Specification
    Left Ventricle
    Permeabilization
    Yes - 0.3% Triton X-100, 10mg/mL BSA, 5% Normal Goat Serum, in PBS
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 29 2014

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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