Recombinant
RabMAb

Recombinant Anti-TIMP1 antibody [EPR1550] - BSA and Azide free (ab240504)

Overview

  • Product name

    Anti-TIMP1 antibody [EPR1550] - BSA and Azide free
    See all TIMP1 primary antibodies
  • Description

    Rabbit monoclonal [EPR1550] to TIMP1 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human TIMP1 aa 50-150.

  • Positive control

    • IHC-P: Human liver, thyroid carcinoma and prostatic hyperplasia tissue.
  • General notes

    ab240504 is the carrier-free version of ab109125 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab240504 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab240504 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 23 kDa.
IHC-P Use at an assay dependent concentration.

See IHC antigen retrieval protocols.

For unpurified use at 1/250 - 1/500.

  • Application notes
    Is unsuitable for Flow Cyt,ICC or IP.
  • Target

    • Function

      Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Also mediates erythropoiesis in vitro; but, unlike IL-3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-11, MMP-12, MMP-13 and MMP-16. Does not act on MMP-14.
    • Sequence similarities

      Belongs to the protease inhibitor I35 (TIMP) family.
      Contains 1 NTR domain.
    • Post-translational
      modifications

      The activity of TIMP1 is dependent on the presence of disulfide bonds.
    • Cellular localization

      Secreted.
    • Information by UniProt
    • Database links

    • Alternative names

      • Clgi antibody
      • Collagenase inhibitor antibody
      • Collagenase inhibitor, Human antibody
      • EPA antibody
      • EPO antibody
      • Erythroid Potentiating Activity antibody
      • Erythroid-potentiating activity antibody
      • Fibroblast collagenase inhibitor antibody
      • FLJ90373 antibody
      • HCI antibody
      • Human Collagenase Inhibitor antibody
      • Metalloproteinase inhibitor 1 antibody
      • Metalloproteinase inhibitor 1 precursor antibody
      • OTTHUMP00000023214 antibody
      • TIMP 1 antibody
      • TIMP antibody
      • TIMP metallopeptidase inhibitor 1 antibody
      • TIMP-1 antibody
      • Timp1 antibody
      • TIMP1 protein antibody
      • TIMP1_HUMAN antibody
      • Tissue Inhibitor of Metalloproteinase 1 antibody
      • Tissue inhibitor of metalloproteinases 1 antibody
      • Tissue inhibitor of metalloproteinases antibody
      • Ttissue inhibitor of metalloproteinase 1 erythroid potentiating activity collagenase inhibitor antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostatic hyperplasia tissue sections labeling TIMP1 with purified ab109125 at 1:8000 dilution (0.21 μg/ml). Heat mediated antigen retrieval was performed using EDTA buffer, pH 9.0. Tissue was counterstained with hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab109125)

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human thyroid carcinoma tissue sections labeling TIMP1 with purified ab109125 at 1:8000 dilution (0.21 μg/ml). Heat mediated antigen retrieval was performed using EDTA buffer, pH 9.0. Tissue was counterstained with hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab109125)

    • Unpurified ab109125, at 1/250 dilution staining TIMP1 in paraffin-embedded human liver tissue, by Immunohistochemistry.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab109125)

    References

    ab240504 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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