Product nameAnti-TIMP1 antibody [EPR16616]
See all TIMP1 primary antibodies
DescriptionRabbit monoclonal [EPR16616] to TIMP1
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse
Recombinant fragment within Mouse TIMP1 aa 1 to the C-terminus. The exact sequence is proprietary.
Database link: P12032
- WB: Mouse lung culture supernatant (7 days) and leg bone lysate.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab179580 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 23 kDa.|
FunctionComplexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Also mediates erythropoiesis in vitro; but, unlike IL-3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-11, MMP-12, MMP-13 and MMP-16. Does not act on MMP-14.
Sequence similaritiesBelongs to the protease inhibitor I35 (TIMP) family.
Contains 1 NTR domain.
modificationsThe activity of TIMP1 is dependent on the presence of disulfide bonds.
- Information by UniProt
- Clgi antibody
- Collagenase inhibitor antibody
- Collagenase inhibitor, Human antibody
All lanes : Anti-TIMP1 antibody [EPR16616] (ab179580) at 1/1000 dilution
Lane 1 : Mouse lung culture supernatant (7 days) at 20 µl
Lane 2 : Mouse leg bone lysate at 10 µg
Lane 1 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Lane 2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 23 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted?
Blocking / Dilution: 5% NFDM/TBST
Exposure time: Lane 1: 32 seconds; Lane 2: 3 minutes
The molecular mass observed is consistent with the literature (PMID: 17202148).
ab179580 has not yet been referenced specifically in any publications.