Product nameAnti-TIMP1 antibody [EPR16616] - BSA and Azide free
See all TIMP1 primary antibodies
DescriptionRabbit monoclonal [EPR16616] to TIMP1 - BSA and Azide free
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse
Recombinant fragment within Mouse TIMP1 aa 1 to the C-terminus. The exact sequence is proprietary.
Database link: P12032
- WB: Mouse lung culture supernatant (7 days) and leg bone lysate.
Ab232937 is the carrier-free version of ab179580. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab232937 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferConstituent: PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab232937 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 23 kDa.|
FunctionComplexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Also mediates erythropoiesis in vitro; but, unlike IL-3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-11, MMP-12, MMP-13 and MMP-16. Does not act on MMP-14.
Sequence similaritiesBelongs to the protease inhibitor I35 (TIMP) family.
Contains 1 NTR domain.
modificationsThe activity of TIMP1 is dependent on the presence of disulfide bonds.
- Information by UniProt
- Clgi antibody
- Collagenase inhibitor antibody
- Collagenase inhibitor, Human antibody
All lanes : Anti-TIMP1 antibody [EPR16616] - BSA and Azide free (ab232937) at 1/1000 dilution
Lane 1 : Mouse lung culture supernatant (7 days) at 20 µl
Lane 2 : Mouse leg bone lysate at 10 µg
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 23 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted?
Blocking / Dilution: 5% NFDM/TBST
Exposure time: Lane 1: 32 seconds; Lane 2: 3 minutes
The molecular mass observed is consistent with the literature (PMID: 17202148).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab179580).
ab232937 has not yet been referenced specifically in any publications.