Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16616] to TIMP1 - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse
Product nameAnti-TIMP1 antibody [EPR16616] - BSA and Azide free
See all TIMP1 primary antibodies
DescriptionRabbit monoclonal [EPR16616] to TIMP1 - BSA and Azide free
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse
Recombinant fragment within Mouse TIMP1 aa 1 to the C-terminus. The exact sequence is proprietary.
Database link: P12032
- WB: Mouse lung culture supernatant (7 days) and leg bone lysate.
Ab232937 is the carrier-free version of ab179580. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab232937 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Storage bufferpH: 7.2
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab232937 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 23 kDa.|
FunctionComplexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Also mediates erythropoiesis in vitro; but, unlike IL-3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-11, MMP-12, MMP-13 and MMP-16. Does not act on MMP-14.
Sequence similaritiesBelongs to the protease inhibitor I35 (TIMP) family.
Contains 1 NTR domain.
modificationsThe activity of TIMP1 is dependent on the presence of disulfide bonds.
- Information by UniProt
- Clgi antibody
- Collagenase inhibitor antibody
- Collagenase inhibitor, Human antibody
All lanes : Anti-TIMP1 antibody [EPR16616] - BSA and Azide free (ab232937) at 1/1000 dilution
Lane 1 : Mouse lung culture supernatant (7 days) at 20 µl
Lane 2 : Mouse leg bone lysate at 10 µg
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 23 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted?
Blocking / Dilution: 5% NFDM/TBST
Exposure time: Lane 1: 32 seconds; Lane 2: 3 minutes
The molecular mass observed is consistent with the literature (PMID: 17202148).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab179580).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab232937 has not yet been referenced specifically in any publications.