Anti-TIMP1 antibody [RM0136-6A34] (ab86482)
Key features and details
- Rat monoclonal [RM0136-6A34] to TIMP1
- Suitable for: IHC-Fr, WB
- Reacts with: Mouse
- Isotype: IgG2
Overview
-
Product name
Anti-TIMP1 antibody [RM0136-6A34]
See all TIMP1 primary antibodies -
Description
Rat monoclonal [RM0136-6A34] to TIMP1 -
Host species
Rat -
Tested applications
Suitable for: IHC-Fr, WBmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant full length Mouse protein
-
Positive control
- WB: Mouse embryonic tissue
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Constituent: PBS -
Concentration information loading...
-
Purification notes
The IgG fraction of culture supernatant was purified by protein A/G affinity chromatography and 0.2µm filtered. -
Clonality
Monoclonal -
Clone number
RM0136-6A34 -
Isotype
IgG2 -
Research areas
Associated products
-
Compatible Secondaries
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab86482 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-Fr |
Use at an assay dependent concentration. PubMed: 21652631
|
|
WB |
1/500 - 1/1000. Predicted molecular weight: 23 kDa.
|
Notes |
---|
IHC-Fr
Use at an assay dependent concentration. PubMed: 21652631 |
WB
1/500 - 1/1000. Predicted molecular weight: 23 kDa. |
Target
-
Function
Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Also mediates erythropoiesis in vitro; but, unlike IL-3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-11, MMP-12, MMP-13 and MMP-16. Does not act on MMP-14. -
Sequence similarities
Belongs to the protease inhibitor I35 (TIMP) family.
Contains 1 NTR domain. -
Post-translational
modificationsThe activity of TIMP1 is dependent on the presence of disulfide bonds. -
Cellular localization
Secreted. - Information by UniProt
-
Database links
- Entrez Gene: 21857 Mouse
- SwissProt: P12032 Mouse
- Unigene: 8245 Mouse
-
Alternative names
- Clgi antibody
- Collagenase inhibitor antibody
- Collagenase inhibitor, Human antibody
see all
Images
Protocols
Datasheets and documents
-
Datasheet download
References (11)
ab86482 has been referenced in 11 publications.
- Klepfish M et al. LOXL2 Inhibition Paves the Way for Macrophage-Mediated Collagen Degradation in Liver Fibrosis. Front Immunol 11:480 (2020). PubMed: 32296422
- Xia F et al. Hsa_circ_0011385 accelerates the progression of thyroid cancer by targeting miR-361-3p. Cancer Cell Int 20:49 (2020). PubMed: 32082079
- Pyka-Fosciak G et al. Effect of natalizumab treatment on metalloproteinases and their inhibitors in a mouse model of multiple sclerosis. J Physiol Pharmacol 71:N/A (2020). PubMed: 32776909
- Yan A et al. CD73 Promotes Glioblastoma Pathogenesis and Enhances Its Chemoresistance via A2B Adenosine Receptor Signaling. J Neurosci 39:4387-4402 (2019). PubMed: 30926752
- Chen H et al. Thalidomide Prevented and Ameliorated Pathogenesis of Crohn's Disease in Mice via Regulation of Inflammatory Response and Fibrosis. Front Pharmacol 10:1486 (2019). PubMed: 31920668